中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2014年
5期
428-433
,共6页
孙敏%张皑峰%杨朝阳%李晓光
孫敏%張皚峰%楊朝暘%李曉光
손민%장애봉%양조양%리효광
创伤性脑损伤%神经营养因子3壳聚糖支架%神经前体细胞%增殖%分化%突触%神经网络
創傷性腦損傷%神經營養因子3殼聚糖支架%神經前體細胞%增殖%分化%突觸%神經網絡
창상성뇌손상%신경영양인자3각취당지가%신경전체세포%증식%분화%돌촉%신경망락
traumatic brain injury%neurotrophin-3 chitosan scaffolds%neural progenitor cells%proliferation%differentiation%synapses%neural network
目的:观察神经营养因子3(NT-3)壳聚糖支架诱导神经突触形成,修复成年大鼠创伤性脑损伤。方法60只成年雄性Wistar大鼠平均分为单纯损伤组、单纯壳聚糖支架组和NT-3壳聚糖支架组,分别于术后3 d、7 d、14 d、28 d和60 d,通过免疫组织化学方法检测损伤区神经再生。术后30 d和60 d应用神经示踪方法与免疫电镜技术观察损伤区内再生的神经突触。结果 NT-3壳聚糖支架组海马损伤区内nestin+、微管蛋白β-tubulin-Ⅲ+、微管相关蛋白2(MAP2)+神经细胞较单纯壳聚糖支架组和单纯损伤组明显增加(P<0.01)。NT-3壳聚糖支架组在海马损伤区内观察到5-溴脱氧尿嘧啶(BrdU)+/MAP2+双阳性新生神经元,并形成突触联系。结论 NT-3壳聚糖支架可激活脑损伤区神经前体细胞增殖,分化为成熟神经元并形成神经突触,参与脑神经网络的重建。
目的:觀察神經營養因子3(NT-3)殼聚糖支架誘導神經突觸形成,脩複成年大鼠創傷性腦損傷。方法60隻成年雄性Wistar大鼠平均分為單純損傷組、單純殼聚糖支架組和NT-3殼聚糖支架組,分彆于術後3 d、7 d、14 d、28 d和60 d,通過免疫組織化學方法檢測損傷區神經再生。術後30 d和60 d應用神經示蹤方法與免疫電鏡技術觀察損傷區內再生的神經突觸。結果 NT-3殼聚糖支架組海馬損傷區內nestin+、微管蛋白β-tubulin-Ⅲ+、微管相關蛋白2(MAP2)+神經細胞較單純殼聚糖支架組和單純損傷組明顯增加(P<0.01)。NT-3殼聚糖支架組在海馬損傷區內觀察到5-溴脫氧尿嘧啶(BrdU)+/MAP2+雙暘性新生神經元,併形成突觸聯繫。結論 NT-3殼聚糖支架可激活腦損傷區神經前體細胞增殖,分化為成熟神經元併形成神經突觸,參與腦神經網絡的重建。
목적:관찰신경영양인자3(NT-3)각취당지가유도신경돌촉형성,수복성년대서창상성뇌손상。방법60지성년웅성Wistar대서평균분위단순손상조、단순각취당지가조화NT-3각취당지가조,분별우술후3 d、7 d、14 d、28 d화60 d,통과면역조직화학방법검측손상구신경재생。술후30 d화60 d응용신경시종방법여면역전경기술관찰손상구내재생적신경돌촉。결과 NT-3각취당지가조해마손상구내nestin+、미관단백β-tubulin-Ⅲ+、미관상관단백2(MAP2)+신경세포교단순각취당지가조화단순손상조명현증가(P<0.01)。NT-3각취당지가조재해마손상구내관찰도5-추탈양뇨밀정(BrdU)+/MAP2+쌍양성신생신경원,병형성돌촉련계。결론 NT-3각취당지가가격활뇌손상구신경전체세포증식,분화위성숙신경원병형성신경돌촉,삼여뇌신경망락적중건。
Objective To repair the traumatic brain injury in adult rats by inducing neural synapses formation with neurotrophin-3 (NT-3) chitosan scaffolds. Methods 60 adult male Wistar rats were equally divided into lesion group, blank chitosan scaffolds group and NT-3 chitosan scaffolds group. The neural regeneration in the lesion area were observed through immunochemistry 3 days, 7 days, 14 days, 28 days, 60 days after operation. Regenerated neural synapses involved the neural circuitry reconstruction in the lesion area were observed through neural tracing and immune electron microscopy 30 days and 60 days after operation. Results The nestin+,β-tubulin-Ⅲ+, microtubule associated protein 2 (MAP2)+neural cells in hippocampal lesion area were significantly more in the NT-3 chitosan scaffolds group than in the other groups (P<0.01). Newborn neurons that express 5-bromouracil deoxyriboside (BrdU) and MAP2 were observed and formed synap-tic connections in hippocampal damage zone in the NT-3 chitosan scaffolds group. Regenerated neural synapses involved the neural circuit-ry reconstruction in the lesion area. Conclusion NT-3 chitosan scaffolds activate the neural progenitor cells to proliferate and differentiate to mature neurons, which form neural synapses to involve the neural circuitry reconstruction.