CAJ | 학술논문

高浓度游离氨(FA)或游离亚硝酸(FNA)条件下硝化过程常出现亚硝态氮积累,FA、FNA 对亚硝态氮氧化菌(NOB)的影响并不清楚.首先用高浓度亚硝态氮污水富集培养 NOB,对富含 NOB 的污泥进行荧光原位杂交技术(FISH)分析表明,Nitrobacter 占细菌总数比例为(71±5)%.用此污泥考察不同 FA、FNA 浓度对 NOB 活性的影响.结果表明,NOB 的活性随着 FA 浓度的增大逐渐减小,当 FA 浓度在10mgNH3-N/L左右时,NOB的活性仅为FA为0时的50%.低浓度的FNA(FNA<0.03mg HNO2-N/L)对NOB活性具有促进作用；当FNA≥0.2mg/L时,NOB的活性被完全抑制.采用Aiba模型计算得到FNA对NOB的抑制常数KI,FNA,NOB为0.0968mg/L. FNA在0.0968mg/L左右时NOB活性仅为FNA为0.003mg/L时的50%.
고농도유리안(FA)혹유리아초산(FNA)조건하초화과정상출현아초태담적루,FA、FNA 대아초태담양화균(NOB)적영향병불청초.수선용고농도아초태담오수부집배양 NOB,대부함 NOB 적오니진행형광원위잡교기술(FISH)분석표명,Nitrobacter 점세균총수비례위(71±5)%.용차오니고찰불동 FA、FNA 농도대 NOB 활성적영향.결과표명,NOB 적활성수착 FA 농도적증대축점감소,당 FA 농도재10mgNH3-N/L좌우시,NOB적활성부위FA위0시적50%.저농도적FNA(FNA<0.03mg HNO2-N/L)대NOB활성구유촉진작용；당FNA≥0.2mg/L시,NOB적활성피완전억제.채용Aiba모형계산득도FNA대NOB적억제상수KI,FNA,NOB위0.0968mg/L. FNA재0.0968mg/L좌우시NOB활성부위FNA위0.003mg/L시적50%.
Nitrite accumulation always accompanies with the nitrification process under the conditions of high concentration of free ammonia (FA) or free nitrous acid (FNA). The impact of FA and FNA concentration on nitrite-oxidising bacteria (NOB) was not clear. In this study, NOB activated sludge was enriched in a lab-scale SBR system with high concentration of nitrite nitrogen wastewater. The fluorescence in situ hybridization (FISH) analysis of NOB-rich sludge showed that Nitrobacter accounted for (71 ± 5)% of total bacteria. The effects of different FA, FNA concentrations on NOB activity were investigated using the sludge. Results showed that NOB activity decreased gradually with the increase of FA concentration. The NOB was still oxidizing nitrite at half of the maximum activity (FA=0mgNH3-N/L) at an FA concentration of 10mgNH3-N/L. Low FNA concentration (FNA<0.03 mg HNO2-N/L) was beneficial to enhance NOB activity and it was completely restrained when FNA≥ 0.2mg/L. The inhibition constant of FNA to NOB (KI,FNA,NOB ) was 0.0968mg/L with Aiba model. The activity of NOB decreased 50% as the FNA concentration increased from 0.003mg/L to 0.0968mg/L.