南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2014年
5期
699-703
,共5页
硫化氢%结肠癌%SW480%增殖%迁移
硫化氫%結腸癌%SW480%增殖%遷移
류화경%결장암%SW480%증식%천이
hydrogen sulfide%human colon cancer%SW480%proliferation%migration
目的:研究H2S对人结肠癌SW480细胞增殖和迁移的作用,及其调控的分子机制。方法用不同浓度H2S处理结肠癌SW480后,采用MTT法检测结肠癌SW480细胞增殖能力的改变,细胞划痕实验观察SW480细胞迁移能力的改变,Western blot实验检测MMP-2、MMP-9和SIRT1表达水平的改变。结果MTT实验,24、48、72 h 3个时间组50、100、200、400μmol/L H2S处理后,与相应时间对照组相比,H2S均能促进细胞的增值;细胞迁移实验结果显示NaHS(100μmol/l)处理组迁移能力增强且明显高于正常对照组(P<0.01),Western blot结果显示NaHS(100μmol/l)处理组MMP-2和MMP-9的表达水平增强且明显高于正常对照组(P<0.05,0.01),且NaHS(100μmol/l)处理组SIRT1表达水平增强。结论H2S能够促进人结肠癌SW480细胞的增殖和迁移,其机制可能与上调SIRT1的表达有关。
目的:研究H2S對人結腸癌SW480細胞增殖和遷移的作用,及其調控的分子機製。方法用不同濃度H2S處理結腸癌SW480後,採用MTT法檢測結腸癌SW480細胞增殖能力的改變,細胞劃痕實驗觀察SW480細胞遷移能力的改變,Western blot實驗檢測MMP-2、MMP-9和SIRT1錶達水平的改變。結果MTT實驗,24、48、72 h 3箇時間組50、100、200、400μmol/L H2S處理後,與相應時間對照組相比,H2S均能促進細胞的增值;細胞遷移實驗結果顯示NaHS(100μmol/l)處理組遷移能力增彊且明顯高于正常對照組(P<0.01),Western blot結果顯示NaHS(100μmol/l)處理組MMP-2和MMP-9的錶達水平增彊且明顯高于正常對照組(P<0.05,0.01),且NaHS(100μmol/l)處理組SIRT1錶達水平增彊。結論H2S能夠促進人結腸癌SW480細胞的增殖和遷移,其機製可能與上調SIRT1的錶達有關。
목적:연구H2S대인결장암SW480세포증식화천이적작용,급기조공적분자궤제。방법용불동농도H2S처리결장암SW480후,채용MTT법검측결장암SW480세포증식능력적개변,세포화흔실험관찰SW480세포천이능력적개변,Western blot실험검측MMP-2、MMP-9화SIRT1표체수평적개변。결과MTT실험,24、48、72 h 3개시간조50、100、200、400μmol/L H2S처리후,여상응시간대조조상비,H2S균능촉진세포적증치;세포천이실험결과현시NaHS(100μmol/l)처리조천이능력증강차명현고우정상대조조(P<0.01),Western blot결과현시NaHS(100μmol/l)처리조MMP-2화MMP-9적표체수평증강차명현고우정상대조조(P<0.05,0.01),차NaHS(100μmol/l)처리조SIRT1표체수평증강。결론H2S능구촉진인결장암SW480세포적증식화천이,기궤제가능여상조SIRT1적표체유관。
Objective To investigate the effect of hydrogen sulfide (H2S) on the proliferation and migration of human colon cancer SW480 cells and explore its molecular mechanisms. Methods The proliferation of SW480 cells exposed to different concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by Western blotting in the exposed cells. Results Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400μmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 μmol/L significantly promoted the cell migration (P<0.01) and enhanced the cellular expressions of MMP-2 (P<0.05) and MMP-9 (P<0.01) proteins;NaHS exposure (100μmol/L) also resulted in up-regulation of SIRT1 expression in SW480 cells. Conclusion H2S can promote proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.