CAJ | 학술논문

研究了ANAMMOX耦合异养反硝化反应器的启动过程,考察了苯酚浓度对耦合反应器脱氮性能的影响.接种2L(占反应器有效容积的20%)挥发性悬浮固体(MLVSS)为6000mg/L 的 ANAMMOX 颗粒污泥,在 pH7.8、温度为25℃、HRT 为1.5h 的条件下经过86d 的培养,ANAMMOX 耦合异养反硝化启动成功.实验结果表明,在稳定运行阶段,NH4+-N、NO2--N 和 TN 平均去除率分别为85.4%、86.1%和79.9%,TN平均容积负荷和TN平均去除负荷分别为2.63,2.10kg/(m3·d)；ANAMMOX颗粒污泥外面包裹着苯酚反硝化菌；系统内异养反硝化与 ANAMMOX 存在协同和竞争关系.当苯酚浓度≥0.3mmol/L 时,ANAMMOX 菌的活性受到很大抑制,苯酚浓度的升高加剧了苯酚反硝化菌与 ANAMMOX 菌之间的竞争；从脱氮效果及系统稳定两方面综合考虑,当苯酚浓度为0.2mmol/L 时,耦合效果最好,消耗的 NH4+-N、NO2--N与生成的NO3--N之比为1：1.52：0.11.
연구료ANAMMOX우합이양반초화반응기적계동과정,고찰료분분농도대우합반응기탈담성능적영향.접충2L(점반응기유효용적적20%)휘발성현부고체(MLVSS)위6000mg/L 적 ANAMMOX 과립오니,재 pH7.8、온도위25℃、HRT 위1.5h 적조건하경과86d 적배양,ANAMMOX 우합이양반초화계동성공.실험결과표명,재은정운행계단,NH4+-N、NO2--N 화 TN 평균거제솔분별위85.4%、86.1%화79.9%,TN평균용적부하화TN평균거제부하분별위2.63,2.10kg/(m3·d)；ANAMMOX과립오니외면포과착분분반초화균；계통내이양반초화여 ANAMMOX 존재협동화경쟁관계.당분분농도≥0.3mmol/L 시,ANAMMOX 균적활성수도흔대억제,분분농도적승고가극료분분반초화균여 ANAMMOX 균지간적경쟁；종탈담효과급계통은정량방면종합고필,당분분농도위0.2mmol/L 시,우합효과최호,소모적 NH4+-N、NO2--N여생성적NO3--N지비위1：1.52：0.11.
The startup of ANAMMOX coupling heterotrophic denitrifying reactor and the influence of concentration of phenol on the nitrogen removal performance of coupled reactor were investigated. Cultivated with 2L (20%of effective volume of reactor) anaerobic ammonia oxidation granular sludge that the volatile suspended solids (MLVSS) was 6000mg/L, anaerobic ammonia oxidation coupling heterotrophic denitrifying was started up successfully for 86days under the condition of pH 7.8, water temperature 25℃and hydraulic detention time (HRT) 1.5h. The steady phase, the average removal rates of NH4+-N、NO2--N and TN were 85.39%,86.12%and 79.85%respectively, the average volumetric load of total nitrogen and the average removal load of total nitrogen were 2.63kg/(m3·d) and 2.10kg/(m3·d) respectively. ANAMMOX granular sludge was coated with phenol denitrifying bacteria. The relationship between heterotrophic denitrification and anammox was cooperative and competitive in the system. The activity of ANAMMOX bacteria was suppressed strongly when the concentration of phenol≥0.3mmol/L, the competition between phenol denitrifying bacteria and ANAMMOX bacteria was intensified as the increasing of concentration of phenol.When the concentration of phenol was 0.2mmol/L, the denitrification effect of system is best considering from denitrification and the stability of system. The ratio of removed NH4+-N, reomved NO2--N and generated NO3--N was 1:1.52:0.11.