CAJ | 학술논문

目的：探讨桦褐孔菌多糖对异烟肼和利福平合用所致肝损伤小鼠的保护作用及其机制。方法48只小鼠随机分为对照组,模型组,护肝片组,桦褐孔菌多糖低、中和高剂量组共6组,每组8只。除对照组外,其余各组每天采用异烟肼和利福平灌胃造成肝损伤模型,2 h后,对照组和模型组分别予以等体积0.9%氯化钠溶液灌胃,其余各组分别予以护肝片、桦褐孔菌多糖灌胃,连续2周。分别检测各组小鼠肝指数和血清丙氨酸氨基转移酶( ALT)、天冬氨酸氨基转移酶( AST)的活性,肝组织匀浆丙二醛( MDA)含量及超氧化物歧化酶( SOD)、谷胱甘肽过氧化物酶( GPx)活性,并做肝组织病理学检查。结果桦褐孔菌多糖组和护肝片组肝指数、血清ALT和 AST活性、肝匀浆MDA含量均明显降低,肝匀浆SOD的活性显著提高。桦褐孔菌多糖组肝细胞变性和坏死程度明显减轻,肝组织的病理改变缓解。结论桦褐孔菌多糖对异烟肼和利福平所致肝损伤小鼠具有明显的保护作用,其机制可能与其抗氧化作用有关。
목적：탐토화갈공균다당대이연정화리복평합용소치간손상소서적보호작용급기궤제。방법48지소서수궤분위대조조,모형조,호간편조,화갈공균다당저、중화고제량조공6조,매조8지。제대조조외,기여각조매천채용이연정화리복평관위조성간손상모형,2 h후,대조조화모형조분별여이등체적0.9%록화납용액관위,기여각조분별여이호간편、화갈공균다당관위,련속2주。분별검측각조소서간지수화혈청병안산안기전이매( ALT)、천동안산안기전이매( AST)적활성,간조직균장병이철( MDA)함량급초양화물기화매( SOD)、곡광감태과양화물매( GPx)활성,병주간조직병이학검사。결과화갈공균다당조화호간편조간지수、혈청ALT화 AST활성、간균장MDA함량균명현강저,간균장SOD적활성현저제고。화갈공균다당조간세포변성화배사정도명현감경,간조직적병리개변완해。결론화갈공균다당대이연정화리복평소치간손상소서구유명현적보호작용,기궤제가능여기항양화작용유관。
Objective To study the protective effect and mechanism of Inonotus obliquus polysaccharide on liver injury induced by isoniazid( INH) combined with rifampicin( RFP) in mice. Methods Forty-eight mice were randomly divided into six groups ( control, model, huganpian, Inonotus obliquus polysaccharide at low, middle, and high dose ) . The mice were administered orally with isoniazid and rifampicin simultaneously except the control. After 2 h,the control and model groups were administered with normal saline,and others were treated with huganpian and Inonotus obliquus polysaccharide,respectively,once a day for 2 weeks. The liver index and serum levels of ALT and AST,malondialdehyde(MDA),superoxide dismutase(SOD) and glutathione peroxidase( GPx) were measured. Pathology examination of liver tissue was performed. Results The activities of ALT and AST, liver index, content of MDA in liver homogenate of mice treated with Inonotus obliquus polysaccharide and huganpian decreased obviously, while the liver SOD activity increased. Histopathological exzamination showed that Inonotus obliquus polysaccharide alleviated the degeneration and necrosis of hepatic cells. Conclusion Inonotus obliquus polysaccharide shows protective effects on mice with hepatic toxic injury induced by isoniazid and rifampicin,which maybe due to its antioxidation effect.