CAJ | 학술논문

利用固体、液体培养法测定溶磷真菌PFK-1的解磷能力；通过盆栽试验，研究其对油菜的促生作用及对土壤磷素变化的影响。试验结果表明，以Ca3（PO4）2为唯一磷源的液体培养基，接种参试菌株PFK-1，在28℃、120 r/min条件下培养144 h溶磷量高达768．1 mg/L；液体培养基中pH值随溶磷量的增加而下降，在144 h时液体培养基pH值降至1．87，以后仍继续保持在低pH值水平；盆栽试验表明，与单施硝基肥的处理相比，1％、2％微生物与硝基肥复配的处理，油菜鲜重和干重增长率分别为7．19％、22．15％和33．11％、40．88％；土壤有效磷分别增加了30．27％、52．36％。
이용고체、액체배양법측정용린진균PFK-1적해린능력；통과분재시험，연구기대유채적촉생작용급대토양린소변화적영향。시험결과표명，이Ca3（PO4）2위유일린원적액체배양기，접충삼시균주PFK-1，재28℃、120 r/min조건하배양144 h용린량고체768．1 mg/L；액체배양기중pH치수용린량적증가이하강，재144 h시액체배양기pH치강지1．87，이후잉계속보지재저pH치수평；분재시험표명，여단시초기비적처리상비，1％、2％미생물여초기비복배적처리，유채선중화간중증장솔분별위7．19％、22．15％화33．11％、40．88％；토양유효린분별증가료30．27％、52．36％。
The phosphate-dissolving ability of phosphate-solubilizing fungi PFK-1 was determined by solid and liquid culture methods.Its growth-promoting effect on rape and impacts on changes of soil phos-phorus were studied by pot experiment.The strain PFK-1 was inoculated in liquid medium with Ca3 (PO4 )2 as the only P source and the phosphorus-dissolving capacity reached 768 mg/L after cultured under condi-tions of 28℃and 120 r/min for 144 hours.The pH in liquid medium decreased with the increase of phosphor-us-dissolving capacity,and reached 1 .87 after cultured for 144 hours and continued to maintain a low level. Pot experiment showed that 1%and 2%PFK-1 compounded with nitro fertilizer could increase the rape fresh weight by 7.19%,22.15% and dry weight by 33.1 1%,40.88% respectively,and the soil available phos-phorus increased by 30.27% and 52.36%.