CAJ | 학술논문

采用HPLC分别测定了橙色果肉甜瓜Homoka和对照白色果肉甜瓜M01-3六个发育时期的β-胡萝卜素及叶黄素含量，并对相关基因作了生物信息学及表达分析。结果表明：随果实发育，橙色甜瓜β-胡萝卜素含量显著升高，在接近成熟时达到积累高峰，成熟时又有所降低；两种甜瓜果实中β-胡萝卜素合成相关基因PSY2、PDS、ZDS、LCY-b的表达量均升高，但橙色甜瓜中PDS和LCY-b表达量高于白色甜瓜；β-胡萝卜素的裂解酶基因CCD1在橙色甜瓜中表达下调，而在白色甜瓜中上调。与白色果肉甜瓜M01-3相比， PDS、LCY-b的高表达和CCD1表达的下调可能决定了橙色甜瓜果实中β-胡萝卜素的高积累量。
채용HPLC분별측정료등색과육첨과Homoka화대조백색과육첨과M01-3륙개발육시기적β-호라복소급협황소함량，병대상관기인작료생물신식학급표체분석。결과표명：수과실발육，등색첨과β-호라복소함량현저승고，재접근성숙시체도적루고봉，성숙시우유소강저；량충첨과과실중β-호라복소합성상관기인PSY2、PDS、ZDS、LCY-b적표체량균승고，단등색첨과중PDS화LCY-b표체량고우백색첨과；β-호라복소적렬해매기인CCD1재등색첨과중표체하조，이재백색첨과중상조。여백색과육첨과M01-3상비， PDS、LCY-b적고표체화CCD1표체적하조가능결정료등색첨과과실중β-호라복소적고적루량。
The HPLC method was used to determine the contents ofβ-carotene and lutein at six devel-opmental stages in orange-fleshed muskmelon Homoka with white-fleshed muskmelon M01 -3 as control. And the bioinformatics and expression analyses of related genes were made.The results showed that the con-tent ofβ-carotene in orange-fleshed muskmelon increased significantly with the development of fruits and reached the accumulation peak when close to maturity,but declined somewhat in mature fruit.The expression level of genes related to biosynthesis ofβ-carotene,PSY2,PDS,ZDS,LCY-b ,were all up-regulated in two muskmelon fruits.But the expression quantity of PDS and LCY-b in orange -fleshed muskmelon was higher than that of white-flashed muskmelon.The expression level of lyase gene ofβ-carotene,CCD1 ,de-scended in orange-fleshed muskmelon,while ascended in white muskmelon.Compared with white -flashed muskmelon,the high expression level of PDS and LCY-b and down-regulated expression of CCD1 possibly determined the high accumulation ofβ-carotene in orange-fleshed muskmelon fruits.