中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
8期
1427-1431
,共5页
田芙颖%甘辉梅%彭南妮%甘菲菲%田菁燕%镇万华%段永刚%蔡志明
田芙穎%甘輝梅%彭南妮%甘菲菲%田菁燕%鎮萬華%段永剛%蔡誌明
전부영%감휘매%팽남니%감비비%전정연%진만화%단영강%채지명
多囊卵巢综合征%树突细胞%免疫耐受%细胞因子类
多囊卵巢綜閤徵%樹突細胞%免疫耐受%細胞因子類
다낭란소종합정%수돌세포%면역내수%세포인자류
Polycystic ovary syndrome%Dendritic cells%Immune tolerance%Cytokines
目的：研究树突状细胞及其细胞因子在多囊卵巢综合征患者在卵泡液中的表达及其临床意义。方法收集多囊卵巢综合征患者取卵日卵泡标本30例，以因男方因素行体外受精-胚胎移植30例患者作为对照组；应用流式细胞术检测树突状细胞的分布，酶联免疫吸附试验检测细胞因子TNF-α、IL-6、IL-10和IL-23等表达水平，并分析HLA-DR平均荧光强度、TNF-α浓度与血雌二醇水平的关系。结果与对照组标本（21.27%±5.5%）相比，多囊卵巢综合征患者CD11c+HLA-DR+DC细胞显著减少（16.22%±5.5%）；hCG日血雌二醇水平与DC活化标志HLA-DR平均荧光强度呈正相关（r＝0.75，P＜0.01），与卵泡液中TNF-α浓度水平呈负相关（r＝-0.69，P＜0.01）。与正常对照组相比，PCOS患者卵泡液TNF-α、IL-6和IL-10的浓度显著增多，而致炎性细胞因子IL-23浓度显著降低。结论 PCOS患者卵泡微环境呈现免疫抑制状态，与卵泡发育障碍可能具有相关性。
目的：研究樹突狀細胞及其細胞因子在多囊卵巢綜閤徵患者在卵泡液中的錶達及其臨床意義。方法收集多囊卵巢綜閤徵患者取卵日卵泡標本30例，以因男方因素行體外受精-胚胎移植30例患者作為對照組；應用流式細胞術檢測樹突狀細胞的分佈，酶聯免疫吸附試驗檢測細胞因子TNF-α、IL-6、IL-10和IL-23等錶達水平，併分析HLA-DR平均熒光彊度、TNF-α濃度與血雌二醇水平的關繫。結果與對照組標本（21.27%±5.5%）相比，多囊卵巢綜閤徵患者CD11c+HLA-DR+DC細胞顯著減少（16.22%±5.5%）；hCG日血雌二醇水平與DC活化標誌HLA-DR平均熒光彊度呈正相關（r＝0.75，P＜0.01），與卵泡液中TNF-α濃度水平呈負相關（r＝-0.69，P＜0.01）。與正常對照組相比，PCOS患者卵泡液TNF-α、IL-6和IL-10的濃度顯著增多，而緻炎性細胞因子IL-23濃度顯著降低。結論 PCOS患者卵泡微環境呈現免疫抑製狀態，與卵泡髮育障礙可能具有相關性。
목적：연구수돌상세포급기세포인자재다낭란소종합정환자재란포액중적표체급기림상의의。방법수집다낭란소종합정환자취란일란포표본30례，이인남방인소행체외수정-배태이식30례환자작위대조조；응용류식세포술검측수돌상세포적분포，매련면역흡부시험검측세포인자TNF-α、IL-6、IL-10화IL-23등표체수평，병분석HLA-DR평균형광강도、TNF-α농도여혈자이순수평적관계。결과여대조조표본（21.27%±5.5%）상비，다낭란소종합정환자CD11c+HLA-DR+DC세포현저감소（16.22%±5.5%）；hCG일혈자이순수평여DC활화표지HLA-DR평균형광강도정정상관（r＝0.75，P＜0.01），여란포액중TNF-α농도수평정부상관（r＝-0.69，P＜0.01）。여정상대조조상비，PCOS환자란포액TNF-α、IL-6화IL-10적농도현저증다，이치염성세포인자IL-23농도현저강저。결론 PCOS환자란포미배경정현면역억제상태，여란포발육장애가능구유상관성。
Objective To investigate the expression of dendritic cells (DC) in follicular fluid of polycystic ovarian syndrome (PCOS) patients and its clinical significance. Methods The samples of follicular fluid were collected from PCOS patients at the day of transvaginal oocyte retrieval (n=30). Patients with severe male factor infertility were set as control group (n=30). Phenotypes of DC were detected by flow cytometer and cytokines (TNF-α, IL-6, IL-10 and IL-23) in follicular fluid were assessed by the enzyme-linked immunosorbent assay (ELISA). Results A significant decrease of the percentage (16.22%±5.5%) of DC was found in PCOS follicular fluid compared with control (21.27%±5.5%). The mean fluorescence intensity of HLA-DR, which reflects DC maturation, correlated positively with serum level of E2 on the day of hCG administration (r=0.75, P<0.01). Serum E2 has a reverse correlation with the concentration of TNF-α in follicular fluid (r=-0.69, P<0.01). The level of cytokines (TNF-α, IL-6 and IL-10) increased significantly but proinflammatory cytokine IL-23 decrease in follicular fluid. The percentage of blood Treg cells increased significantly in carcinoma group compared with normal group but less than that of cancerous tissues in bladder carcinoma. Conclusion The decrease of follicular fluid DC from PCOS patients indicates an immunosuppressive regulatory profile in the microenvironment of ovarian follicle, which is associated with the dysfunction of folliculogenesis.
