CAJ | 학술논문

采用表面受限光接枝技术在玻璃表面构筑末端酯键可水解的羧酸甜菜碱酯阳离子聚合物刷( PCBMA-1C2),通过调节氨水浓度控制羧酸甜菜碱酯末端酯键的水解程度,实现表面聚合物刷中季铵阳离子和羧酸甜菜碱两性离子基团分配比例的改变.通过X射线光电子能谱( XPS)和接触角测试表征了改性表面的化学结构和亲/疏水性能,通过蛋白质吸附和血小板黏附实验研究了玻璃表面改性前后及水解前后电荷性质对生物分子相互作用的影响.结果发现,当氨水浓度为0.1,0.2,0.3和0.4 mol/L时,聚合物刷PCBMA-1C2改性表面羧酸甜菜碱酯末端酯键的水解率分别为6%,43%,56%和~100%,随着水解程度的增大,改性表面牛血清白蛋白(BSA)的吸附量依次降低了3%,76%,93%,96%,纤维蛋白原(Fg)吸附量则依次降低了11%,45%,90%和96%；当水解率>50%时,改性表面表现出优异的抗蛋白质吸附和血小板黏附性能.
채용표면수한광접지기술재파리표면구축말단지건가수해적최산첨채감지양리자취합물쇄( PCBMA-1C2),통과조절안수농도공제최산첨채감지말단지건적수해정도,실현표면취합물쇄중계안양리자화최산첨채감량성리자기단분배비례적개변.통과X사선광전자능보( XPS)화접촉각측시표정료개성표면적화학결구화친/소수성능,통과단백질흡부화혈소판점부실험연구료파리표면개성전후급수해전후전하성질대생물분자상호작용적영향.결과발현,당안수농도위0.1,0.2,0.3화0.4 mol/L시,취합물쇄PCBMA-1C2개성표면최산첨채감지말단지건적수해솔분별위6%,43%,56%화~100%,수착수해정도적증대,개성표면우혈청백단백(BSA)적흡부량의차강저료3%,76%,93%,96%,섬유단백원(Fg)흡부량칙의차강저료11%,45%,90%화96%；당수해솔>50%시,개성표면표현출우이적항단백질흡부화혈소판점부성능.
A hydrolysable cationic polycarboxybetaine ester polymer brush ( PCBMA-1 C2 ) was fabricated by photografting method with surface-coupled tertiary amine as hetergenous hydrogen donor and benzophenone (BP) as photosensitizer. The hydrolysis of PCBMA-1C2 modified surface was evaluated by studying the hydrolysis behavior of CBMA-1C2 monomer under different ammonium hydroxide concentrations. X-ray photoelectron spectroscopy ( XPS ) and water contact angle were used to monitor the chemical changes and hydrophilicity, respectively. The protein adsorption and platelet adhesion experiments were used to evaluate the interaction between the material surface and the biomolecules. The results show that when the concentra-tions of ammonium hydroxide are 0. 1, 0. 2, 0. 3 and 0. 4 mol/L, the degrees of hydrolysis( DH) of terminal ester of CBMA-1C2 monomer are 6%, 43%, 56% and almost 100%, respectively. Compared with Glass-PCBMA-1C2 surface, with the increase of DH, the protein adsorption of BSA are reduced by 3%, 76%, 93% and 96%, and that of Fg are reduced by 11%, 45%, 90% and 96%, respectively. When DH>50%, the hydrolyzed Glass-PCBMA-1 C2 surface exhibits good resistance of protein adsorption and platelet adhesion.