CAJ | 학술논문

根据植物中CAD基因保守序列设计引物，以尾叶桉GLU4嫩茎为材料克隆到其CAD基因，命名为EuCAD。序列分析结果表明，该基因gDNA长2200 bp， cDNA长1102 bp，开放阅读区编码356个氨基酸。EuCAD编码的蛋白质序列分析显示，序列中具有完整CAD1保守结构域，并包含NADP结合位点以及2个Zn结合位点。同源性分析结果显示， EuCAD核酸序列与其他5种桉属植物CAD基因同源性达到97％以上， EuCAD编码蛋白与碧桃等植物中CAD蛋白同源性也在79％以上。对EuCAD编码蛋白进行理化性质及结构的生物信息学分析结果显示，该蛋白为一种无信号肽、无跨膜区的酸性蛋白质，二级结构包含典型的α-螺旋和β-折叠，三级结构同源建模显示与拟南芥的AtCAD4同源性极高。系统进化树分析结果显示，该蛋白与桉属、杨属植物亲缘关系较近，与杉科植物较远。
근거식물중CAD기인보수서렬설계인물，이미협안GLU4눈경위재료극륭도기CAD기인，명명위EuCAD。서렬분석결과표명，해기인gDNA장2200 bp， cDNA장1102 bp，개방열독구편마356개안기산。EuCAD편마적단백질서렬분석현시，서렬중구유완정CAD1보수결구역，병포함NADP결합위점이급2개Zn결합위점。동원성분석결과현시， EuCAD핵산서렬여기타5충안속식물CAD기인동원성체도97％이상， EuCAD편마단백여벽도등식물중CAD단백동원성야재79％이상。대EuCAD편마단백진행이화성질급결구적생물신식학분석결과현시，해단백위일충무신호태、무과막구적산성단백질，이급결구포함전형적α-라선화β-절첩，삼급결구동원건모현시여의남개적AtCAD4동원성겁고。계통진화수분석결과현시，해단백여안속、양속식물친연관계교근，여삼과식물교원。
A CAD gene was cloned from the young stem of Eucalyptus urophylla clone GLU4 and named EuCAD by using specific primers based on the highly conserved sequences of plant CAD.Sequence analysis indicated that its gDNA and cDNA sequence were 2 200 bp and 1 102 bp respectively , which open reading frame encoding 356 ami-no acid residues .EuCAD encoding sequence was analyzed , and the result showed it has an entire CAD 1 domain, a NADP binding site and two Zn binding sites .The results of homology analysis showed that EuCAD nucleotide se-quence had more than 97%sequence homology with that of other 5 Eucalyptus, and EuCAD encoding protein had more than 79 %homology with that of Prunus persica and others.The physicochemical property and structure of EuCAD encoding protein were analyzed using bioinformatics tools .The result showed it was an acidic protein with-out signal peptide or transmembrane domain , and its secondary structure has typical α-helix and β-sheet.Tertiary structure modeling showed it was most similar to AtCAD 4 in Arabidopsis thaliana.Phylogenetic analysis showed that it has close relative relation with Eucalyptus and Populus, but distant relation with Taxodiaceae .