CAJ | 학술논문

目的：HCV胶体金法与ELISA法用于输血前检查丙型肝炎抗体检测的应用效果比较。方法：分别采用ELISA法和胶体金法对我院576名受血者的血清样本进行HCV-抗体检测，对两种检测方法的检测效果及检测用时及成本预算进行综合比较。结果：ELISA法阳性检测率高于胶体金法，但比较差异无统计学意义（P>0.05）；经胶体金法和ELISA检测为阳性的血清样本再使用RT-PCR 荧光定量法检测，胶体金法的检测符合率达到100%，显著高于ELISA法（P<0.05）；胶体金检测法所需检测时间短且检测所需成本更低。结论：与ELISA法比较，胶体金法用于检测丙型肝炎病毒的假阳性率更低，操作更方便，成本花费低，值得临床推广应用。
목적：HCV효체금법여ELISA법용우수혈전검사병형간염항체검측적응용효과비교。방법：분별채용ELISA법화효체금법대아원576명수혈자적혈청양본진행HCV-항체검측，대량충검측방법적검측효과급검측용시급성본예산진행종합비교。결과：ELISA법양성검측솔고우효체금법，단비교차이무통계학의의（P>0.05）；경효체금법화ELISA검측위양성적혈청양본재사용RT-PCR 형광정량법검측，효체금법적검측부합솔체도100%，현저고우ELISA법（P<0.05）；효체금검측법소수검측시간단차검측소수성본경저。결론：여ELISA법비교，효체금법용우검측병형간염병독적가양성솔경저，조작경방편，성본화비저，치득림상추엄응용。
Objective:HCV colloidal gold method and ELISA method is used to check the application effect of hepatitis c antibody testing before blood transfusion. Methods:the ELISA method and colloidal gold method a total of 576 recipients serum samples of HCV antibody detection, two test methods for the detection effect of the comprehensive comparison and testing time and cost budget.Results:the method of ELISA positive detection rate is higher than that of colloidal gold method, but there was no statistical significance (P>0.05) difference comparison;The colloidal gold method and ELISA test for positive serum samples using the fluorescent quantitative rt-pcr method to detect, colloidal gold method detection coincidence rate reached 100%, significantly higher than that of ELISA method (P < 0.05); Colloidal gold test required for testing time is short and the cost is lower. Conclusions:compared with ELISA method, colloidal gold method is used to detect lower false positive rate of hepatitis c virus (HCV), operation more convenient, cost is low, worthy of clinical popularization and application.