CAJ | 학술논문

目的：(1)明确2型糖尿病患者和健康人群的脂联素rs+16861194(A/G)位点的三种基因型分布是否有差异；(2)探讨脂联素rs+16861194的基因多态性与T2DM及健康人群部分生化指标水平的关系。方法：选取158例2型糖尿病患者为实验组,132例健康体检人群为对照组。分别对实验组和对照组进行联素基因rs+16861194基因型进行检测；同时检测空腹葡萄糖,甘油三酯(TG)，高密度脂蛋白-胆固醇(HDL)，低密度脂蛋白-胆固醇(LDL)；采用化学发光法测定空腹胰岛素,并计算胰岛素抵抗指数。结果：①T2DM组和CON组脂联素rs+16861194位点的基因型分布分别为：AA(70.00%)、AG(24.37%)、GG(5.63%)和AA(74.24%)、AG(24.24%)、GG(1.52%)；②以脂联素rs+16861194位点的基因型分组，在T2DM组和CON组内，各基因型之间的FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH水平均无统计学差异（P>0.05）。③在T2DM组与CON组之间比较，T2DM组AA型与CON组AA型的FBG，FIN，IRI，TG，HDL差异有统计学意义（P<0.05）；CHOL，LDL差异无统计学意义（P>0.05）。T2DM组AG+GG型与CON组AG+GG型的FBG，IRI，TG，HDL差异有统计学意义（P<0.05）；FIN，CHOL，LOL差异无统计学意义（P>0.05）。结论：（1）脂联素rs+16861194(A/G)位点的三种基因型分布无显著性差异。（2）脂联素rs+16861194的基因型在2型糖尿病人群内或健康人群内对FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH水平无影响。
목적：(1)명학2형당뇨병환자화건강인군적지련소rs+16861194(A/G)위점적삼충기인형분포시부유차이；(2)탐토지련소rs+16861194적기인다태성여T2DM급건강인군부분생화지표수평적관계。방법：선취158례2형당뇨병환자위실험조,132례건강체검인군위대조조。분별대실험조화대조조진행련소기인rs+16861194기인형진행검측；동시검측공복포도당,감유삼지(TG)，고밀도지단백-담고순(HDL)，저밀도지단백-담고순(LDL)；채용화학발광법측정공복이도소,병계산이도소저항지수。결과：①T2DM조화CON조지련소rs+16861194위점적기인형분포분별위：AA(70.00%)、AG(24.37%)、GG(5.63%)화AA(74.24%)、AG(24.24%)、GG(1.52%)；②이지련소rs+16861194위점적기인형분조，재T2DM조화CON조내，각기인형지간적FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH수평균무통계학차이（P>0.05）。③재T2DM조여CON조지간비교，T2DM조AA형여CON조AA형적FBG，FIN，IRI，TG，HDL차이유통계학의의（P<0.05）；CHOL，LDL차이무통계학의의（P>0.05）。T2DM조AG+GG형여CON조AG+GG형적FBG，IRI，TG，HDL차이유통계학의의（P<0.05）；FIN，CHOL，LOL차이무통계학의의（P>0.05）。결론：（1）지련소rs+16861194(A/G)위점적삼충기인형분포무현저성차이。（2）지련소rs+16861194적기인형재2형당뇨병인군내혹건강인군내대FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH수평무영향。
Objective:(1) To explore the difference of three kinds of adiponectin rs +16861194 (A / G ) genotype distribution in the type 2 diabetes patients and healthy people ; (2) To investigate the relationship of adiponectin rs+16861194 gene polymorphism with some biochemical indicators from T2DM and control.Methods:Selected 158 cases of type 2 diabetes patients as the experimental group, 132 cases of healthy people as the control group. The genotypes of adiponectin rs-16861194 in experimental group and the control group were detected; At the same time, fasting plasma glucose, triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) were measured seperately. Fasting insulin was measured by chemiluminescence and insulin resistance index was calculated.Results:①T2DM group and CON group adiponectin rs +16861194 genotype distribution were: AA (70.00%), AG (24.37%), G (5.63%) and AA (74.24%), AG (24.24%), GG (1.52%), the distribution showed no statistically significant difference (P> 0.05); ②Grouped by the genotype of adiponectin rs +16861194, the level of FBG, FIN, IRI, TG, CHOL, HDL-CH, LDL-CH showed no statistically significant difference (P> 0.05) between the genotypes in T2DM and CON group. ③ Compraring between T2DM group and CON group,.the level of FBG, FIN, IRI, TG, HDL in the AA type of T2DM group and CON group had significant difference (P<0.05), while the level of CHOL and LDL showed no statistically significant difference (P> 0.05) ; The level of FBG,IRI,TG,HDL in the AG+GG type of T2DM group and CON group had significant difference (P<0.05), while the level of FIN,CHOL,LOL showed no statistically significant difference (P> 0.05).Conclusions:(1) Three kinds of locus genotype distribution of rs +16861194 (A / G) had no significant difference in Type 2 diabetes patients and healthy people.(2) The genotype of adiponectin rs+16861194 had no effect on the level of FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH in type 2 diabetes patients and healthy people.