CAJ | 학술논문

在培养基中添加浓度为50μmol/L、150μmol/L、200μmol/L、250μmol/L、300μmol/L和400μmol/L的茉莉酸甲酯（MeJA），将分化结束后培养1个月的三七组培苗接种于其中，培养2天，5天，10天，15天，30天，60天时，分别取样，分析三七组培苗中的总皂苷含量，研究不同浓度的茉莉酸甲酯对三七组培苗总皂苷含量的影响。结果表明，三七组培苗在MeJA处理2天，5天，10天，15天，30天后，其总皂苷含量均显著高于对照组，至60天时，浓度为200μmol/L，250μmol/L的MeJA处理的三七组培苗样品中，总皂苷含量显著高于对照组，其他浓度MeJA处理的样品与对照无显著差异。其中以浓度为250μmol/L MeJA处理后第5天的三七组培苗样品的总皂苷含量最高，达到10.05％，是同期对照样品含量的16.8倍，且总皂苷含量呈现先升、后降、最后达到相对平衡的规律。
재배양기중첨가농도위50μmol/L、150μmol/L、200μmol/L、250μmol/L、300μmol/L화400μmol/L적말리산갑지（MeJA），장분화결속후배양1개월적삼칠조배묘접충우기중，배양2천，5천，10천，15천，30천，60천시，분별취양，분석삼칠조배묘중적총조감함량，연구불동농도적말리산갑지대삼칠조배묘총조감함량적영향。결과표명，삼칠조배묘재MeJA처리2천，5천，10천，15천，30천후，기총조감함량균현저고우대조조，지60천시，농도위200μmol/L，250μmol/L적MeJA처리적삼칠조배묘양품중，총조감함량현저고우대조조，기타농도MeJA처리적양품여대조무현저차이。기중이농도위250μmol/L MeJA처리후제5천적삼칠조배묘양품적총조감함량최고，체도10.05％，시동기대조양품함량적16.8배，차총조감함량정현선승、후강、최후체도상대평형적규률。
One-month tissue cultural seedlings of Panax notoginseng with fully differentiated were treated with methy jasmonate (MeJA) at concentrations of 50μmol/L, 150 μmol/L, 200μmol/L, 250 μmol/L, 300 μmol/L and 400 μmol/L, respectively, in order to understand the effects of methy jasmonate on the content of total sapo-nin in tissue culture seedlings of Panax notoginseng, different sampling were made with different MeJA treatment seedlings after 2 days, 5 days, 10 days, 15 days, 30 days and 60 days.The results showed that the contents of total saponin in all the MeJA treatment samples were higher than those in the controls , in 2 days, 5 days, 10 days, 15 days and 30 days after MA treatment .After 60 days, the content of total saponin with treatments of 200μmol/L and 250μmol/L MeJA were still higher than the control , but other treatments had no significant difference with the control.The highest content of total saponin , which was 10.05 % and about 16.8 times of the control , was found in the treatment with 250 μmol/L MeJA, 5 days after the treatment.The total saponin content was in-creased first , then declined and finally reached to a relatively constant level.