CAJ | 학술논문

目的：探讨醋酸纤维素电泳和毛细管电泳在血红蛋白（Hb）E病筛查中的价值差异。方法采用pH8．6醋酸纤维素电泳及SEBIA capillarys2全自动毛细管电泳仪对基因诊断确诊为 HbE阳性的17例地中海贫血患者的血红蛋白组分进行检测。结果17例 HbE阳性患者醋酸纤维素电泳 HbA2＋ HbE吸光度最高值为86．43，最低值为16．95，相应的毛细管电泳HbE＋ HbA2吸光度最高值为91．3（6．8＋84．5），最低值为19．8（3．2＋16．6）；2种方法对 HbE病的诊断结果高度一致，与基因诊断的相符率均为100％。结论醋酸纤维素电泳和毛细管电泳在HbE的筛查中结果一致。
목적：탐토작산섬유소전영화모세관전영재혈홍단백（Hb）E병사사중적개치차이。방법채용pH8．6작산섬유소전영급SEBIA capillarys2전자동모세관전영의대기인진단학진위 HbE양성적17례지중해빈혈환자적혈홍단백조분진행검측。결과17례 HbE양성환자작산섬유소전영 HbA2＋ HbE흡광도최고치위86．43，최저치위16．95，상응적모세관전영HbE＋ HbA2흡광도최고치위91．3（6．8＋84．5），최저치위19．8（3．2＋16．6）；2충방법대 HbE병적진단결과고도일치，여기인진단적상부솔균위100％。결론작산섬유소전영화모세관전영재HbE적사사중결과일치。
Objective To study the value difference of cellulose acetate electrophoresis and capillary electrophoresis in screening for hemoglobin E(HbE) disease .Methods Cellulose acetate electrophoresis and SEBIA capillarys2 automated capillary electropho-resis instrument were employed to detect the hemoglobin components of 17 patients with thalassemia and positive HbE which were confirmed by genetic diagnosis .Results The highest and the lowest absorbance values of HbA2+ HbE of 17 patients with positive HbE according to cellulose acetate electrophoresis were 86 .43 and 16 .95 ,respectively ,while the corresponding absorbance values were 91 .3(6 .8+84 .5) and 19 .8(3 .2+16 .6) according to capillary electrophoresis ,respectively .The diagnostic results of the two methods for HbE were highly consistent and they matched well with genetically diagnostic results with the match rate of 100% . Conclusion Cellulose acetate electrophoresis and capillary electrophoresis have consistent screening results for HbE .