CAJ | 학술논문

目的：基于聚合酶链反应（PCR）-高分辨率熔解（HRM）曲线分析方法和葡萄球菌蛋白A（SPA）分型方法，建立一种适用于耐甲氧西林金黄色葡萄球菌（MRSA）的快速分型方法。方法收集临床分离的71株MRSA作为试验菌株，采用基因测序和HRM曲线分析方法对菌株进行 SPA基因分型。结果采用基因测序方法，71株MRSA菌株的 SPA基因分为4个型别，分别是t570、t030、t002和t588，以t570（53例）为主要型别，其次是t030和t002（均为7例）。HRM分析对 S PA基因的分型结果与基因测序方法的结果基本一致。结论 PCR-HRM分析有望成为一种快速、有效的MRSA的 SPA基因分型方法，为医院感染控制提供依据。
목적：기우취합매련반응（PCR）-고분변솔용해（HRM）곡선분석방법화포도구균단백A（SPA）분형방법，건립일충괄용우내갑양서림금황색포도구균（MRSA）적쾌속분형방법。방법수집림상분리적71주MRSA작위시험균주，채용기인측서화HRM곡선분석방법대균주진행 SPA기인분형。결과채용기인측서방법，71주MRSA균주적 SPA기인분위4개형별，분별시t570、t030、t002화t588，이t570（53례）위주요형별，기차시t030화t002（균위7례）。HRM분석대 S PA기인적분형결과여기인측서방법적결과기본일치。결론 PCR-HRM분석유망성위일충쾌속、유효적MRSA적 SPA기인분형방법，위의원감염공제제공의거。
Objective To establish a rapid genotyping method of for methicillin-resistant Staphylococcus aureus(MRSA) based on polymerase chain reaction(PCR)-high resolution melting (HRM ) curve analysis and staphylococcal protein A (SPA ) classifica-tion .Methods 71 strains of MRSA clinically isolated were collected as test strains .Gene sequencing and HRM curve analysis were employed to conduct SPA gene typing .Results According to gene sequencing method ,SPA gene of 71 strains of MRSA was divided into four types ,namely t570 ,t030 ,t002 and t588 .The most predominant type was t570 (74 .65% ) ,followed by t030 and t002(both 7 cases) .The result of SPA gene typing by HRM analysis were basically consistent with that by gene sequencing .Con-clusion PCR-HRM analysis is expected to become a fast ,efficient genotyping for MRSA SPA gene ,providing the basis for hospital infection control .