CAJ | 학술논문

目的：研究TMEM135 mRNA及蛋白在人体多种正常及肿瘤组织的表达。方法原位RT-PCR及免疫组化技术检测TMEM135在正常组织118例、良性肿瘤组织18例、恶性肿瘤组织55例中的表达。结果 TMEM135 mRNA和蛋白在人体组织中普遍表达；TMEM135 mRNA主要分布在核膜上，而TMEM135蛋白主要分布在胞质，且多为颗粒状分布；正常组织与恶性肿瘤间TMEM135蛋白表达有统计学差异（P＜0．05）。结论 TMEM135可能是一种与肿瘤相关的蛋白质。
목적：연구TMEM135 mRNA급단백재인체다충정상급종류조직적표체。방법원위RT-PCR급면역조화기술검측TMEM135재정상조직118례、량성종류조직18례、악성종류조직55례중적표체。결과 TMEM135 mRNA화단백재인체조직중보편표체；TMEM135 mRNA주요분포재핵막상，이TMEM135단백주요분포재포질，차다위과립상분포；정상조직여악성종류간TMEM135단백표체유통계학차이（P＜0．05）。결론 TMEM135가능시일충여종류상관적단백질。
Objective To study the expression of TMEM 135 mRNA and protein in a variety of normal and tumor tis-sues.Methods We used in situ RT-PCR and immunohistochemistry to detect the expression of TMEM 135 mRNA and protein in 118 cases of normal tissues , 18 cases of benign and 55 cases of malignant tissues .Results TMEM135 mRNA and protein expressed widely in the human tissues .TMEM135 mRNA mainly distributed in the nuclear membrane and the protein was mainly in the cytoplasm which was mostly grainy distributed .There was significant difference in the expression of TMEM135 protein between normal and cancer tissues .Conclusion TMEM135 might be a protein which is associated with the tumor .