CAJ | 학술논문

目的：复制帕金森病（Parkinson?s disease，PD）大鼠模型并根据行为学检查结果对此模型进行较为全面的评价，以期建立治疗此疾病的新型实验平台。方法：运用6-羟多巴胺（6-hydroxydopamine）单点定向注射黑质-纹状体的方法，损毁SD大鼠左侧中脑多巴胺能神经元，动物术后3周腹腔注射（intrapertioneal injection，ip）阿扑吗啡（apomorphine，APO）观察是否诱导动物向健侧旋转行为，复制PD模型。分别应用转棒实验和旷场实验分析测定动物的运动功能，应用肌电测试实验测定大鼠的震颤频率和幅度，并据此对动物模型进行评价和分类。结果：造模后，部分大鼠转棒实验在棒时间显著缩短，旷场实验横向跨格次数减少，出现运动功能障碍；肌电检测结果显示部分大鼠肌肉震颤频率和幅度均增加。结论：行为学测试为PD动物模型的评价以及进行不同类型的分组提供了实验依据。
목적：복제파금삼병（Parkinson?s disease，PD）대서모형병근거행위학검사결과대차모형진행교위전면적평개，이기건립치료차질병적신형실험평태。방법：운용6-간다파알（6-hydroxydopamine）단점정향주사흑질-문상체적방법，손훼SD대서좌측중뇌다파알능신경원，동물술후3주복강주사（intrapertioneal injection，ip）아복마배（apomorphine，APO）관찰시부유도동물향건측선전행위，복제PD모형。분별응용전봉실험화광장실험분석측정동물적운동공능，응용기전측시실험측정대서적진전빈솔화폭도，병거차대동물모형진행평개화분류。결과：조모후，부분대서전봉실험재봉시간현저축단，광장실험횡향과격차수감소，출현운동공능장애；기전검측결과현시부분대서기육진전빈솔화폭도균증가。결론：행위학측시위PD동물모형적평개이급진행불동류형적분조제공료실험의거。
Objective:To systematically evaluate 6-hydroxydopamine(6-OHDA)-induced behavioral alteration as a rat model of Parkinson’s disease. Methods:6-OHDA was microinjected into the left side of the substantia nigra striatum to damage the dopaminergic neurons in the SD rats. Three weeks later,intraperitoneal injection of apomorphine(APO)to observe the rotational behavior. The motoric function of animals was analyzed with rotarod test and open field test, and the rat’s muslce vibration frequency and amplitude were determined using the myoelectricity test. The severity of the behavioral alterations of the individual animals was also categorized. Results:The time of rats that remained on the rotarod was significantly reduced in model group as compared to sham group. In the open field test,the horizontal travel distance was decreased in the model group. The myoelectricity test result showed that the muscle vibration frequency and amplitude was increased in animals receiving 6-OHDA microinjection. Conclusion:These results provided behavioral evidence in future studies to evaluate and categorize Parkinson-like behaviors in rats.