CAJ | 학술논문

本研究采用反转录多聚酶链式反应（ RT-PCR）的方法对野外采集的河北种群淡色库蚊的钠离子通道基因进行了克隆和序列分析。得到的克隆序列与GenBank中公布的序列能够吻合，说明成功克隆出淡色库蚊的钠通道基因全长，为抗性突变的研究奠定了基础。本研究在淡色库蚊钠通道基因上发现了多处氨基酸突变（A32T、 T336A、 S342P、 K535E、 L1035F、 N1595S和F1982L）和7处可变剪接。其中，包括经典的抗性突变L1014F （ L1035F）。但这些突变和可变剪接与蚊虫抗性的关系有待进一步研究。
본연구채용반전록다취매련식반응（ RT-PCR）적방법대야외채집적하북충군담색고문적납리자통도기인진행료극륭화서렬분석。득도적극륭서렬여GenBank중공포적서렬능구문합，설명성공극륭출담색고문적납통도기인전장，위항성돌변적연구전정료기출。본연구재담색고문납통도기인상발현료다처안기산돌변（A32T、 T336A、 S342P、 K535E、 L1035F、 N1595S화F1982L）화7처가변전접。기중，포괄경전적항성돌변L1014F （ L1035F）。단저사돌변화가변전접여문충항성적관계유대진일보연구。
The sodium channel gene of Culex pipiens pallens collected from Hebei Province was cloned and sequenced using reverse transcription polymerase chain reaction ( RT-PCR ) .The alignment results are consistent with the sodium channel gene announced in GenBank.The study lays a foundation for development of the researches on mosquito resistance. Meanwhile, seven amino acid substitutions ( A32T, T336A, S342P, K535E, L1035F, N1595S and F1982L) and seven alternative splicing have been found in the sodium channel gene from this field Cx.pipiens pallens, including the classic resistant mutation L1014F ( L1035F).The relationship between these substitutions and resistance is still unknown and deserve further study.