中国感染与化疗杂志
中國感染與化療雜誌
중국감염여화료잡지
CHINESE JOURNAL OF INFECTION AND CHEMOTHERAPY
2014年
2期
142-145
,共4页
邹自英%韩黎%熊杰%卢中一%孟祥兆%张雨龙%赵静雅%韩雪琳%田曙光%陈勇
鄒自英%韓黎%熊傑%盧中一%孟祥兆%張雨龍%趙靜雅%韓雪琳%田曙光%陳勇
추자영%한려%웅걸%로중일%맹상조%장우룡%조정아%한설림%전서광%진용
金黄色葡萄球菌%spa分型%耐药性%感染控制
金黃色葡萄毬菌%spa分型%耐藥性%感染控製
금황색포도구균%spa분형%내약성%감염공제
Staphylococcus aureus%spa typing%antibiotic resistance%infection control
目的：研究临床分离金葡菌的耐药性和分子特征。方法对2011年5-11月在成都某医院分离的56株金葡菌进行抗菌药物敏感性检测，检测菌株携带 mecA 基因和 pvl 基因情况，并进行 spa 基因分型分析。结果56株金葡菌中，共检出耐甲氧西林金葡菌（MRSA）21株（37．5％），其中 mecA 基因阳性20株（95．2％）；甲氧西林敏感金葡菌（MSSA）35株（62．5％）。与 MSSA 相比，MRSA 对利福平、左氧氟沙星、莫西沙星、环丙沙星、庆大霉素和四环素的敏感率显著降低（P ＜0．05），MRSA对万古霉素、利奈唑胺、替加环素、喹奴普丁-达福普汀和呋喃妥因全部敏感。MRSA 分为6个 spa 型别，以 t030（66．7％）为主，MRSA-t030、MRSA-t002具有突出的多药耐药特征。MSSA 共分为18个 spa 型别，以 t189、t377和 t034列前3位，分别占14．3％、14．3％和11．4％，并在脓液标本检出1株新 spa 基因型 new1。检出5株杀白细胞毒素（PVL）阳性菌株，其中3株为MSSA-t189。结论t030是医院 MRSA 临床分离株中的 spa 优势型别，具有突出的多药耐药特征，在医院内广泛传播。MSSA 遗传多样性高，以 t189、t377和 t034列前3位。
目的：研究臨床分離金葡菌的耐藥性和分子特徵。方法對2011年5-11月在成都某醫院分離的56株金葡菌進行抗菌藥物敏感性檢測，檢測菌株攜帶 mecA 基因和 pvl 基因情況，併進行 spa 基因分型分析。結果56株金葡菌中，共檢齣耐甲氧西林金葡菌（MRSA）21株（37．5％），其中 mecA 基因暘性20株（95．2％）；甲氧西林敏感金葡菌（MSSA）35株（62．5％）。與 MSSA 相比，MRSA 對利福平、左氧氟沙星、莫西沙星、環丙沙星、慶大黴素和四環素的敏感率顯著降低（P ＜0．05），MRSA對萬古黴素、利奈唑胺、替加環素、喹奴普丁-達福普汀和呋喃妥因全部敏感。MRSA 分為6箇 spa 型彆，以 t030（66．7％）為主，MRSA-t030、MRSA-t002具有突齣的多藥耐藥特徵。MSSA 共分為18箇 spa 型彆，以 t189、t377和 t034列前3位，分彆佔14．3％、14．3％和11．4％，併在膿液標本檢齣1株新 spa 基因型 new1。檢齣5株殺白細胞毒素（PVL）暘性菌株，其中3株為MSSA-t189。結論t030是醫院 MRSA 臨床分離株中的 spa 優勢型彆，具有突齣的多藥耐藥特徵，在醫院內廣汎傳播。MSSA 遺傳多樣性高，以 t189、t377和 t034列前3位。
목적：연구림상분리금포균적내약성화분자특정。방법대2011년5-11월재성도모의원분리적56주금포균진행항균약물민감성검측，검측균주휴대 mecA 기인화 pvl 기인정황，병진행 spa 기인분형분석。결과56주금포균중，공검출내갑양서림금포균（MRSA）21주（37．5％），기중 mecA 기인양성20주（95．2％）；갑양서림민감금포균（MSSA）35주（62．5％）。여 MSSA 상비，MRSA 대리복평、좌양불사성、막서사성、배병사성、경대매소화사배소적민감솔현저강저（P ＜0．05），MRSA대만고매소、리내서알、체가배소、규노보정-체복보정화부남타인전부민감。MRSA 분위6개 spa 형별，이 t030（66．7％）위주，MRSA-t030、MRSA-t002구유돌출적다약내약특정。MSSA 공분위18개 spa 형별，이 t189、t377화 t034렬전3위，분별점14．3％、14．3％화11．4％，병재농액표본검출1주신 spa 기인형 new1。검출5주살백세포독소（PVL）양성균주，기중3주위MSSA-t189。결론t030시의원 MRSA 림상분리주중적 spa 우세형별，구유돌출적다약내약특정，재의원내엄범전파。MSSA 유전다양성고，이 t189、t377화 t034렬전3위。
Objective To study the resistance and molecular profiles of Staphylococcus aureus strains isolated from the clinical specimens.Methods Antimicrobial susceptibility was tested with 56 strains of Staphylococcus aureus isolated from a hospital from May to November 2011.The mecA and pvl genes were detected.The spa genetic types were analyzed.Results A total of 21 (37.5%)Staphylococcus aureus strains were resistant to methicillin (MRSA)and 35 (62.5%)were sensitive to methicillin (MSSA).Nineteen of the 21 (90.5%)MRSA strains carried mecA gene.Compared with MSSA,MRSA were much less sen-sitive to rifampin,fluoroqunolones,tetracycline and gentamicin (P <0.05).All the MRSA isolates were susceptible to vanco-mycin,linezolid,tigecycline,quinupristin-dalfopristin and nitrofurantoin.Six spa types were identified among the MRSA strains.Type t030 was the most prevalent,accounting for 66.7% (14/21)of all the MRSA strains.MRSA-t030 and MRSA-t002 were resistant to multiple antibiotics.Eighteen spa types were identified among the MSSA strains.Type t189,t377 and t034 were the top three spa types of MSSA,accounting for 14.3%,14.3% and 11.4%,respectively.A new MSSA spa typ-ing strain new1 was isolated from pus.There were five Panton-Valentine leukocidin (PVL)-positive isolates,3 of which was MSSA-t189 type. Conclusions Type t030 is the most prevalent spa type among clinical MRSA strains,which is resistant to many kinds of antibiotics and widely spreads in the hospital setting.There are many different spa types a-mong the MSSA strains.Type t389,t377 and t034 are the top three spa types of MSSA.
