中国临床保健杂志
中國臨床保健雜誌
중국림상보건잡지
CHINESE JOURNAL OF CLINICAL HEALTHCARE
2014年
3期
280-283,337
,共5页
程民%陈稳%毛菊%王静%程翠%沈国栋%卞庚%徐婷娟%徐维平%沈干%胡世莲
程民%陳穩%毛菊%王靜%程翠%瀋國棟%卞庚%徐婷娟%徐維平%瀋榦%鬍世蓮
정민%진은%모국%왕정%정취%침국동%변경%서정연%서유평%침간%호세련
免疫疗法%细胞培养技术%细胞因子诱导杀伤细胞%卵巢肿瘤%疾病模型,动物
免疫療法%細胞培養技術%細胞因子誘導殺傷細胞%卵巢腫瘤%疾病模型,動物
면역요법%세포배양기술%세포인자유도살상세포%란소종류%질병모형,동물
Immunotherapy%Cell culture techniques%Cytokine-induced killer cells%Ovarian neoplasms%Dis-ease Models,Animal
目的：建立培养细胞因子诱导的杀伤（CIK）细胞方法，并评价其基本特点、体内外抗肿瘤活性及安全性。方法使用抗CD3单克隆抗体、rhIL-2及rhIFN-γ刺激PBMC细胞，使其在体外大量扩增，利用流式细胞术、体外杀伤实验及荷人卵巢癌小鼠模型检测CIK细胞的特点及抗肿瘤活性；并检测CIK细胞中外源性因子污染情况及其急性毒性作用。结果体外培养21 d后，CIK细胞总数可达1010以上，其主要成分为CD3＋CD8＋T细胞及CD3＋CD56＋NKT细胞，CIK细胞在体外对Ho8910、A549、K562及HepG2细胞均有较高的杀伤活性，并对荷瘤小鼠具有治疗作用。同时在CIK细胞中未检测到外源性病原体污染。结论 CIK细胞在体内外均具有较高的抗肿瘤活性且无毒性作用。
目的：建立培養細胞因子誘導的殺傷（CIK）細胞方法，併評價其基本特點、體內外抗腫瘤活性及安全性。方法使用抗CD3單剋隆抗體、rhIL-2及rhIFN-γ刺激PBMC細胞，使其在體外大量擴增，利用流式細胞術、體外殺傷實驗及荷人卵巢癌小鼠模型檢測CIK細胞的特點及抗腫瘤活性；併檢測CIK細胞中外源性因子汙染情況及其急性毒性作用。結果體外培養21 d後，CIK細胞總數可達1010以上，其主要成分為CD3＋CD8＋T細胞及CD3＋CD56＋NKT細胞，CIK細胞在體外對Ho8910、A549、K562及HepG2細胞均有較高的殺傷活性，併對荷瘤小鼠具有治療作用。同時在CIK細胞中未檢測到外源性病原體汙染。結論 CIK細胞在體內外均具有較高的抗腫瘤活性且無毒性作用。
목적：건립배양세포인자유도적살상（CIK）세포방법，병평개기기본특점、체내외항종류활성급안전성。방법사용항CD3단극륭항체、rhIL-2급rhIFN-γ자격PBMC세포，사기재체외대량확증，이용류식세포술、체외살상실험급하인란소암소서모형검측CIK세포적특점급항종류활성；병검측CIK세포중외원성인자오염정황급기급성독성작용。결과체외배양21 d후，CIK세포총수가체1010이상，기주요성분위CD3＋CD8＋T세포급CD3＋CD56＋NKT세포，CIK세포재체외대Ho8910、A549、K562급HepG2세포균유교고적살상활성，병대하류소서구유치료작용。동시재CIK세포중미검측도외원성병원체오염。결론 CIK세포재체내외균구유교고적항종류활성차무독성작용。
Objective To establish the methods of cytokine-induced killer (CIK)cells and evaluate the characteristics,anti-tumor activity in vitro and in vivo and the safety.Methods Anti-CD3 mAb,recombinant human interleukin 2 (rhIL-2 )and recombinant human interferon-γ(rhIFN-γ)were used to induce the peripheral blood mononuclear cells (PBMCs)proliferation abundantly in vitro.Flow cytometry assay (FACS),in vitro cytotoxicity as-say and tumor mouse model xenografted with Ho-8910 (human ovarian cancer cell line)were used to detect the char-acteristics and the anti-tumor activityof CIK cells.Additionally,the contamination of exogenous agents and the acute toxicity effect of CIK cells were also determined.Results After 21 days in vitro culture,the total number of CIK cells were more than 1010,among which there were mainly CD3 +CD8 +T cells and CD3 +CD56 +NKT cells.CIK cells dis-played high cytotoxicity against Ho8910,A549,K562 and HepG2 cells in vitro,and they also showed therapeutic effects in tumor xenografted mice.Additionally,no acute toxicity was observed in CIK cells-treated mice,and there was also no contamination of bacteria,fungus,mycoplasma and virus in CIK cells.Conclusion CIK cells have high anti-tumor activity without any toxic side effects.
