中国临床保健杂志
中國臨床保健雜誌
중국림상보건잡지
CHINESE JOURNAL OF CLINICAL HEALTHCARE
2014年
3期
276-280,337
,共6页
哈德丽娅·加帕尔%伊力多斯·艾合他木夫%阿依甫汗·阿汗
哈德麗婭·加帕爾%伊力多斯·艾閤他木伕%阿依甫汗·阿汗
합덕려아·가파이%이력다사·애합타목부%아의보한·아한
糖尿病,1 型%牙龈炎%白细胞介素2%白细胞介素4%细粒棘球绦虫%疾病模型,动物
糖尿病,1 型%牙齦炎%白細胞介素2%白細胞介素4%細粒棘毬縚蟲%疾病模型,動物
당뇨병,1 형%아간염%백세포개소2%백세포개소4%세립극구조충%질병모형,동물
Diabetes Mellitus,Type 1%Gingivitis%Interleukin-2%Interleukin-4%Echinococcus granulosus%Dis-ease Models,Animal
目的：研究细粒棘球蚴B抗原（EgB）对链脲佐菌素（STZ）诱导的1型糖尿病（T1 DM）模型小鼠牙龈组织中白细胞介素-2（IL -2）及白细胞介素-4（IL -4）表达产生的影响。方法雌性10周龄STZ 诱导的T1DM模型小鼠20只，随机分为2组，每组10只，处理组给予EgB 100μg/10 g连续5 d腹腔注射，对照组给予同等剂量0．9％氯化钠注射溶液腹腔注射，取其牙龈组织，用荧光定量聚合酶链反应（FQ-PCR）检测牙龈中IL-2、IL-4 mRNA表达。结果 IL-2 mRNA在EgB处理组较对照组表达明显下降（P＜0．05）；IL-4 mRNA在EgB处理组较对照组表达明显上升（P＜0．05）。结论 EgB处理STZ 诱导的T1 DM模型小鼠，使其牙龈中IL-2 mRNA表达降低，IL-4 mRNA表达升高，影响T1 DM免疫应答机制。
目的：研究細粒棘毬蚴B抗原（EgB）對鏈脲佐菌素（STZ）誘導的1型糖尿病（T1 DM）模型小鼠牙齦組織中白細胞介素-2（IL -2）及白細胞介素-4（IL -4）錶達產生的影響。方法雌性10週齡STZ 誘導的T1DM模型小鼠20隻，隨機分為2組，每組10隻，處理組給予EgB 100μg/10 g連續5 d腹腔註射，對照組給予同等劑量0．9％氯化鈉註射溶液腹腔註射，取其牙齦組織，用熒光定量聚閤酶鏈反應（FQ-PCR）檢測牙齦中IL-2、IL-4 mRNA錶達。結果 IL-2 mRNA在EgB處理組較對照組錶達明顯下降（P＜0．05）；IL-4 mRNA在EgB處理組較對照組錶達明顯上升（P＜0．05）。結論 EgB處理STZ 誘導的T1 DM模型小鼠，使其牙齦中IL-2 mRNA錶達降低，IL-4 mRNA錶達升高，影響T1 DM免疫應答機製。
목적：연구세립극구유B항원（EgB）대련뇨좌균소（STZ）유도적1형당뇨병（T1 DM）모형소서아간조직중백세포개소-2（IL -2）급백세포개소-4（IL -4）표체산생적영향。방법자성10주령STZ 유도적T1DM모형소서20지，수궤분위2조，매조10지，처리조급여EgB 100μg/10 g련속5 d복강주사，대조조급여동등제량0．9％록화납주사용액복강주사，취기아간조직，용형광정량취합매련반응（FQ-PCR）검측아간중IL-2、IL-4 mRNA표체。결과 IL-2 mRNA재EgB처리조교대조조표체명현하강（P＜0．05）；IL-4 mRNA재EgB처리조교대조조표체명현상승（P＜0．05）。결론 EgB처리STZ 유도적T1 DM모형소서，사기아간중IL-2 mRNA표체강저，IL-4 mRNA표체승고，영향T1 DM면역응답궤제。
Objective To study the effect of Echinococcus antigen B(EgB)on the expression of IL-2 and IL-4 in the gingival tissues of streptozotocin (STZ)-induced type 1 diabetic mouse model.Methods A total of 20 ten-week-old female STZ-induced type 1 diabetic mouse were randomly divided into 2 groups,with 10 mouse in each group.The mouse in the treatment group was injected intraperitonealy with EgB (100μg/10g)for 5 consecutive days, and the mouse in he control group were injected with 0.9% sodium chloride with the same dose.The gingival tissues were collected,and the expression IL-2,IL-4 mRNA was determined by FQ-PCR.Result The level of IL-2 mRNA in the treatment group was significantly lower than thant in the control group(P<0.05);The level of IL-4 mRNA in the EgB treated group was significantly increased compared with the control group(P<0.05).Conclusion The expres-sion of IL -2 is decreased and the level of IL-4 is increased in the gingival tissues of streptozotocin (STZ)-induced type 1 diabetic mouse model treated with EgB.
