CAJ | 학술논문

目的：探讨老年创伤患者外周血中髓源抑制细胞（ MDSCs ）比例变化及其临床意义。方法收集26例青年创伤患者（18～30岁）和21例老年创伤患者（＞60岁），以CD14-/CD11b＋/CD33＋作为MD-SCs标志物，流式细胞术（FCM）检测MDSCs比例，酶联免疫吸附试验（ELISA）检测血清白介素-10、转化生长因子-β和C反应蛋白水平，分析MDSCs比例的变化情况及其临床意义。结果伤后1d，老年组和青年组创伤患者外周血MDSCs比例无显著差异（P＞0.05）。伤后3d和7d，老年组创伤患者外周血MDSCs比例均高于青年组，差异有显著意义（ P＜0.01）。 MDSCs比例和血清转化生长因子-β、白介素-10和C反应蛋白水平均有相关性。结论老年创伤患者外周血MDSCs比例较青年组显著增高，可能是老年创伤患者更容易发生免疫抑制的重要机制。
목적：탐토노년창상환자외주혈중수원억제세포（ MDSCs ）비례변화급기림상의의。방법수집26례청년창상환자（18～30세）화21례노년창상환자（＞60세），이CD14-/CD11b＋/CD33＋작위MD-SCs표지물，류식세포술（FCM）검측MDSCs비례，매련면역흡부시험（ELISA）검측혈청백개소-10、전화생장인자-β화C반응단백수평，분석MDSCs비례적변화정황급기림상의의。결과상후1d，노년조화청년조창상환자외주혈MDSCs비례무현저차이（P＞0.05）。상후3d화7d，노년조창상환자외주혈MDSCs비례균고우청년조，차이유현저의의（ P＜0.01）。 MDSCs비례화혈청전화생장인자-β、백개소-10화C반응단백수평균유상관성。결론노년창상환자외주혈MDSCs비례교청년조현저증고，가능시노년창상환자경용역발생면역억제적중요궤제。
Objective To explore the variation and significance of CD 14 -/CD11b+/CD33 +myeloid de-rived suppressor cells ( MDSCs ) in elderly patients with multiple injuries . Methods Totally 26 young patients ( age 18-30 years ) and 21 elderly patients ( age >60 years ) with multiple injury were enrolled in this study . CD14 -/CD11b+/CD33 +MDSCs in peripheral blood were determined by flow cytometry ( FCM) at 1,3 and 7days after trauma.Serum transforming growth factor-β(TGF-β),interleukin-10 and C-reactive protein were determined by ELISA.Results The percentages of CD14 -/CD11b+/CD33 +MDSCs in peripheral blood of elderly patients at 3 and 7days after trauma were significantly higher than those of young patients (P<0.01),whereas did not differ significantly at 1 day after trauma between the two groups (P >0.05).The percentages of CD14 -/CD11b+/CD33 +MDSCs in peripheral blood of elderly patients with multiple injury were closely correlated with serum TGF -β,interleukin-10 and C-reactive protein level (P <0.05).Conclusion The percentages of CD14 -/CD11b+/CD33 +MDSCs in peripheral blood were significantly increased in elderly patients with multiple injuries .Our results indicated that accumulation of CD 14 -/CD11b+/CD33 +MDSCs may be an important mechanism of aging-related immune dysfunction in elderly patients with multiple injuries .