CAJ | 학술논문

目的：建立同时测定一枝黄花药材中绿原酸、芦丁和山奈素含量的高效液相色谱法。方法：采用Diamonsil C18柱（4.6 mm×200 mm，5μm），流动相乙腈-0.2%磷酸水溶液梯度洗脱，流速1.0 mL·min-1，柱温25℃，检测波长282 nm，柱温25℃。结果：绿原酸、芦丁和山奈素的回归方程分别为Y=1712.1X+11.686（r=0.9993）、Y=13584.0X+13.984（r=0.9994）、Y=5971.2X+4.6175（r=0.9998）；线性范围分别为63.2~442.4μg、8.1~56.8μg、10.8~75.7μg；回收率分别为98.6%（RSD=1.4%）、99.2%（RSD=0.8%）、100.3%（RSD=1.0%）。结论：本法简便快捷，重复性好，可用于一枝黄花中绿原酸、芦丁和山奈素的定量分析。
목적：건립동시측정일지황화약재중록원산、호정화산내소함량적고효액상색보법。방법：채용Diamonsil C18주（4.6 mm×200 mm，5μm），류동상을정-0.2%린산수용액제도세탈，류속1.0 mL·min-1，주온25℃，검측파장282 nm，주온25℃。결과：록원산、호정화산내소적회귀방정분별위Y=1712.1X+11.686（r=0.9993）、Y=13584.0X+13.984（r=0.9994）、Y=5971.2X+4.6175（r=0.9998）；선성범위분별위63.2~442.4μg、8.1~56.8μg、10.8~75.7μg；회수솔분별위98.6%（RSD=1.4%）、99.2%（RSD=0.8%）、100.3%（RSD=1.0%）。결론：본법간편쾌첩，중복성호，가용우일지황화중록원산、호정화산내소적정량분석。
This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column (4.6 mm í 200 mm, 5 μm) was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2~442.4 μg for chlorogenic acid (r = 0.999 3), 8.1~56.8 μg for rutin (r = 0.999 4) and 10.8~75.7μg for kaempferide (r = 0.999 8). The average recovery of chlorogenic acid was 98.6% (RSD = 1.4%), that of rutin was 99.2% (RSD = 0.8%) and that of kaempferide was 100.3% (RSD = 1.0%). It was concluded that the method was simple, economical and accurate with good reproducibility.