CAJ | 학술논문

目的：研究中药注射剂的过敏反应，测定致敏动物血清免疫球蛋白（IgE）抗体含量，观察其与过敏反应是否具有相关性，为提高过敏反应实验预测的准确性提供实验依据。方法：选用多种中药注射剂进行被动皮肤过敏实验（PCA）实验和类过敏反应研究，用ELISA法对致敏动物血清进行血清IgE水平检测。结果：双黄连注射液（SHL）、清开灵注射液（QKL）、鱼腥草注射液（YXC）、血塞通注射液（XST）、葛根素注射液（GGS）、穿琥宁注射液（CHN）的PCA实验结果均为阴性，刺五加注射液（CWJ）的PCA实验结果为阳性。SHL、QKL、YXC、XST、GGS、CHN、CWJ注射液组血清总 IgE水平与生理盐水组无明显差异；SHL、QKL、YXC、XST、GGS、CHN、CWJ注射液均引起了豚鼠的类过敏症状。结论：SHL、QKL、YXC、XST、GGS、CHN、CWJ均能引起豚鼠的类过敏反应，其过敏反应的发生与血清IgE抗体水平无相关性。
목적：연구중약주사제적과민반응，측정치민동물혈청면역구단백（IgE）항체함량，관찰기여과민반응시부구유상관성，위제고과민반응실험예측적준학성제공실험의거。방법：선용다충중약주사제진행피동피부과민실험（PCA）실험화류과민반응연구，용ELISA법대치민동물혈청진행혈청IgE수평검측。결과：쌍황련주사액（SHL）、청개령주사액（QKL）、어성초주사액（YXC）、혈새통주사액（XST）、갈근소주사액（GGS）、천호저주사액（CHN）적PCA실험결과균위음성，자오가주사액（CWJ）적PCA실험결과위양성。SHL、QKL、YXC、XST、GGS、CHN、CWJ주사액조혈청총 IgE수평여생리염수조무명현차이；SHL、QKL、YXC、XST、GGS、CHN、CWJ주사액균인기료돈서적류과민증상。결론：SHL、QKL、YXC、XST、GGS、CHN、CWJ균능인기돈서적류과민반응，기과민반응적발생여혈청IgE항체수평무상관성。
This study was aimed to investigate allergic reactions of traditional Chinese medicine (TCM) injections, and to determine contents of serum IgE in sensitized animals. The correlation between preceding contents in serum and allergic reactions may be found, in order to offer experimental evidences for advancing the accuracy of anticipa-tion by allergic reactions. Passive cutaneous anaphylaxis (PCA) tests and anaphylactoid reactions were used in a vari-ety of TCM injections. ELISA method was used to determine the content of total serum IgE in sensitized animals. The PCA results of SHL, QKL, YXC, XST, GGS and CHN were negative. The PCA result of CWJ was positive. There was no significant difference for total serum IgE level between the experimental group and the normal saline group in the group of SHL, QKL, YXC, XST, GGS, CHN and CWJ. All TCM injections caused anaphylactoid symp-toms in guinea pigs. It was concluded that all TCM injections can cause allergic reactions in guinea pig. And the al-lergic reactions of TCM injections were not correlated with serum total IgE.