世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
4期
825-829
,共5页
吴立洁%刘杰%王文祎%戴待%程新宇%张子龙%杨瑶珺
吳立潔%劉傑%王文祎%戴待%程新宇%張子龍%楊瑤珺
오립길%류걸%왕문의%대대%정신우%장자룡%양요군
三七%根际土壤%酚酸类物质%含量测定
三七%根際土壤%酚痠類物質%含量測定
삼칠%근제토양%분산류물질%함량측정
Panax notoginseng%rhizosphere soil%phenolic acids%content determination
目的:对云南文山三七根际土壤中酚酸的种类进行鉴定,并对其进行含量测定。方法:采用HPLC-MS、HPLC技术,并通过文献和对照品比对,鉴定了云南文山三七根际土壤中酚酸的种类并对其进行含量测定。结果:从三七根际土壤提取液中检测到对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸和苯甲酸6种酚酸类物质,其中对香豆酸含量最高(24.01μg·g-1),丁香酸含量最低(1.26μg·g-1)。6种酚酸类成分在三七根际土壤中的含量顺序为对香豆酸>香草酸>苯甲酸>对羟基苯甲酸>阿魏酸>丁香酸。结论:三七根际土壤中的酚酸类物质可能为三七植物须根残体腐解产生,也可能是三七根系分泌产生。
目的:對雲南文山三七根際土壤中酚痠的種類進行鑒定,併對其進行含量測定。方法:採用HPLC-MS、HPLC技術,併通過文獻和對照品比對,鑒定瞭雲南文山三七根際土壤中酚痠的種類併對其進行含量測定。結果:從三七根際土壤提取液中檢測到對羥基苯甲痠、香草痠、丁香痠、對香豆痠、阿魏痠和苯甲痠6種酚痠類物質,其中對香豆痠含量最高(24.01μg·g-1),丁香痠含量最低(1.26μg·g-1)。6種酚痠類成分在三七根際土壤中的含量順序為對香豆痠>香草痠>苯甲痠>對羥基苯甲痠>阿魏痠>丁香痠。結論:三七根際土壤中的酚痠類物質可能為三七植物鬚根殘體腐解產生,也可能是三七根繫分泌產生。
목적:대운남문산삼칠근제토양중분산적충류진행감정,병대기진행함량측정。방법:채용HPLC-MS、HPLC기술,병통과문헌화대조품비대,감정료운남문산삼칠근제토양중분산적충류병대기진행함량측정。결과:종삼칠근제토양제취액중검측도대간기분갑산、향초산、정향산、대향두산、아위산화분갑산6충분산류물질,기중대향두산함량최고(24.01μg·g-1),정향산함량최저(1.26μg·g-1)。6충분산류성분재삼칠근제토양중적함량순서위대향두산>향초산>분갑산>대간기분갑산>아위산>정향산。결론:삼칠근제토양중적분산류물질가능위삼칠식물수근잔체부해산생,야가능시삼칠근계분비산생。
Phenolic acids of rhizosphere soil of Panax notoginseng were identified and the contents were determined. HPLC-MS and HPLC were used. Compared with the literature and standard reference, identification and content de-termination were given on phenolic acids of rhizosphere soil of Panax notoginseng in Wenshan, Yunnan province. The results showed that six types of phenolic acids were detected from the extract of rhizosphere soil of Panax notogin-seng, which were p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid and benzoic acid. The content of p-coumaric acid showed the highest concentration of 24.01 μg·g-1, while syringic acid had the lowest concentration of 1.26 μg·g-1. The sequence of content in the rhizosphere soil of Panax notoginseng from top to bot-tom was p-coumaric acid, vanillic acid, benzoic acid, p-hydroxybenzoic acid, ferulic acid and syringic acid. It was concluded that the rhizosphere soil of Panax notoginseng contained six types of phenolic acids, which were p-hy-droxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid and benzoic acid.