CAJ | 학술논문

目的：探讨miR-24-2对人骨肉瘤细胞系U-2OS体外增殖能力的影响。方法用脂质体法将miR-24-2模拟物转染入人骨肉瘤细胞系U-2OS。将U-2OS细胞分为miR-24-2模拟物转染组、miR-24-2抑制剂转染组、阴性对照组和正常对照组4组。转染后采用实时定量RT-PCR检测各组细胞miR-24-2的表达水平，采用MTT法检测各组细胞的增殖情况。结果与阴性对照组和正常对照组比较，miR-24-2模拟物转染组细胞增殖能力显著下降，而miR-24-2抑制剂转染组细胞增殖能力显著增强。结论miR-24-2能够抑制人骨肉瘤细胞系U-2OS的体外增殖能力，可望成为骨肉瘤治疗的分子标志物。
목적：탐토miR-24-2대인골육류세포계U-2OS체외증식능력적영향。방법용지질체법장miR-24-2모의물전염입인골육류세포계U-2OS。장U-2OS세포분위miR-24-2모의물전염조、miR-24-2억제제전염조、음성대조조화정상대조조4조。전염후채용실시정량RT-PCR검측각조세포miR-24-2적표체수평，채용MTT법검측각조세포적증식정황。결과여음성대조조화정상대조조비교，miR-24-2모의물전염조세포증식능력현저하강，이miR-24-2억제제전염조세포증식능력현저증강。결론miR-24-2능구억제인골육류세포계U-2OS적체외증식능력，가망성위골육류치료적분자표지물。
Objective To explore the effect of miR-24-2 on the in vitro proliferation of U-2OS cells. Methods U-2OS cells were randomly allocat-ed into 4 groups:miR-24-2 mimic group,miR-24-2 inhibitor group,negative control group,and normal control group. MicroRNAs were transfected into U-2OS cells using Lipofectamine?2000. miR-24-2 expression level and the proliferation of U-2OS cells after transfection were detected by real-time quantitative RT-PCR(RT-qPCR)and MTT proliferation assays,respectively. Results RT-qPCR results showed that miR-24-2 level was sig-nificantly higher in the miR-24-2 mimic group and lower in the miR-24-2 inhibitor group than those in the controls,indicating the successive trans-fection. MTT proliferation assay results proved that the cell viability was significantly lower in the U-2OS cells transfected with miR-24-2 mimic and higher in inhibitor groups compared to the control. Conclusion MiR-24-2 inhibits growth of the U-2OS cells,which could be a potential biomarker in the treatment of osteosarcoma.