CAJ | 학술논문

目的：探讨微小RNA（miRNA）-1对大鼠心肌细胞凋亡的影响。方法应用转染的方法使培养的大鼠H9c2心肌细胞高表达miRNA-1（miRNA-1组），real-time PCR方法检测miRNA-1表达水平以确定转染成功后，MTT法检测细胞活力，流式细胞术测定细胞凋亡情况，分别采用real-time PCR和Western Blot方法检测凋亡抑制基因Bcl-2的mRNA和蛋白表达情况，并与常规条件下培养的H9c2细胞（空白对照组）和转染随机合成的miRNA阴性对照片段（阴性对照组）比较。结果与空白对照组和阴性对照组相比，转染miRNA-1 mimics后，H9c2大鼠心肌细胞miRNA-1表达水平明显升高，细胞凋亡率显著增加，细胞生存率、Bcl-2的mRNA和蛋白表达水平显著降低。结论转染的miRNA-1 mimics能够上调细胞内的miRNA-1表达水平，抑制心肌细胞增殖，促进细胞凋亡。
목적：탐토미소RNA（miRNA）-1대대서심기세포조망적영향。방법응용전염적방법사배양적대서H9c2심기세포고표체miRNA-1（miRNA-1조），real-time PCR방법검측miRNA-1표체수평이학정전염성공후，MTT법검측세포활력，류식세포술측정세포조망정황，분별채용real-time PCR화Western Blot방법검측조망억제기인Bcl-2적mRNA화단백표체정황，병여상규조건하배양적H9c2세포（공백대조조）화전염수궤합성적miRNA음성대조편단（음성대조조）비교。결과여공백대조조화음성대조조상비，전염miRNA-1 mimics후，H9c2대서심기세포miRNA-1표체수평명현승고，세포조망솔현저증가，세포생존솔、Bcl-2적mRNA화단백표체수평현저강저。결론전염적miRNA-1 mimics능구상조세포내적miRNA-1표체수평，억제심기세포증식，촉진세포조망。
Objective To investigate the effect of miRNA-1 on the cardiomyocyte apoptosis in rats. Methods MicroRNA-1 mimics was transfected into the cultured H9c2 cell line (miRNA-1 group). Cells transfected with random miR-NA fragment was used as negative control group. The cell apoptosis was evaluated by FCM assay. MTT assay was used to de-tect the cell viability. The expression level of miRNA-1 was detected by real-time PCR. The expression levels of Bcl-2 mRNA and protein were detected by real-time PCR and Western blot assay. Results Compared with normal and negative control groups, the expression level of miRNA-1 was significantly higher in H9c2 cardiomyocytes, the apoptosis rate was in-creased, the cell vitality and Bcl-2 expression level were significantly decreased after transfection of miRNA-1 mimics. Conclusion miRNA-1 mimics can up-regulate miRNA-1 level, inhibit proliferation and induce cardiomyocyte apoptosis.