CAJ | 학술논문

目的：探讨不同浓度镁离子对大鼠血管平滑肌细胞(VSMCs)钙化的影响。方法原代培养获取VSMCs，进行形态学及免疫细胞鉴定，后将VSMCs随机分为阴性对照组、高磷组、镁干预组。阴性对照组采用含10%胎牛血清培养，高磷组采用高磷培养基培养，镁干预组在高磷培养基的基础上分别加入不同浓度氯化镁，使镁离子终浓度分别为1、2、3 mmol/L（镁干预组1~3），刺激7 d后行钙化检测，测定钙含量及碱性磷酸酶（ALP）活性，并行反转录聚合酶链反应（RT-PCR）检测细胞内核心结合因子α1（Cbfα1）mRNA的表达。结果高磷组和镁干预组VSMCs均有钙盐沉积，其钙含量均高于阴性对照组；镁干预组随镁离子浓度增大钙化结节逐渐缩小，除镁干预组1钙含量与高磷组差异无统计学意义外，镁干预组2和镁干预组3均低于高磷组（均P＜0.05）。VSMCs ALP活性和Cbfα1 mRNA的表达除镁干预组3与阴性对照组差异无统计学意义外，其余组均高于阴性对照组（P＜0.05）。镁干预组随镁离子浓度增大，ALP活性和Cbfα1 mRNA的表达水平均逐渐降低，且均低于高磷组（P＜0.05）。结论镁离子可在一定程度上抑制高磷诱导的VSMCs钙化和成骨样转分化，其可能是通过降低VSMCs中Cbfα1的表达来实现的。
목적：탐토불동농도미리자대대서혈관평활기세포(VSMCs)개화적영향。방법원대배양획취VSMCs，진행형태학급면역세포감정，후장VSMCs수궤분위음성대조조、고린조、미간예조。음성대조조채용함10%태우혈청배양，고린조채용고린배양기배양，미간예조재고린배양기적기출상분별가입불동농도록화미，사미리자종농도분별위1、2、3 mmol/L（미간예조1~3），자격7 d후행개화검측，측정개함량급감성린산매（ALP）활성，병행반전록취합매련반응（RT-PCR）검측세포내핵심결합인자α1（Cbfα1）mRNA적표체。결과고린조화미간예조VSMCs균유개염침적，기개함량균고우음성대조조；미간예조수미리자농도증대개화결절축점축소，제미간예조1개함량여고린조차이무통계학의의외，미간예조2화미간예조3균저우고린조（균P＜0.05）。VSMCs ALP활성화Cbfα1 mRNA적표체제미간예조3여음성대조조차이무통계학의의외，기여조균고우음성대조조（P＜0.05）。미간예조수미리자농도증대，ALP활성화Cbfα1 mRNA적표체수평균축점강저，차균저우고린조（P＜0.05）。결론미리자가재일정정도상억제고린유도적VSMCs개화화성골양전분화，기가능시통과강저VSMCs중Cbfα1적표체래실현적。
Objective To explore the effects of the different concentrations of magnesium ions on vascular smooth muscle cell (VSMC) calcification in rats. Methods VSMCs were obtained from rat aortic, and were identified by immunocy-tochemistry. VSMCs were then randomly divided into control group, high phosphorus group and magnesium intervention group. VSMCs were cultured with 10%fetal bovine serum in control group. VSMCs were cultured with high phosphorus in high phosphorus group. VSMCs were cultured with different concentrations of magnesium chloride based on the high phos-phorus medium in magnesium intervention group (final concentrations of magnesium ions were 1, 2 and 3 mmol/L). The calci-um content and alkaline phosphatase(ALP)activity were measured after the stimulation for 7 days. The expression of Cbfα1 mRNA was detected by RT-PCR. Results Compared with control group, calcium deposits were found significantly higher in high phosphorus group and magnesium intervention group. The calcified nodules gradually reduced with the increased magnesium ion concentration in the intervention group. The calcium contents were significantly lower in the intervention groups (2 and 3 mmol/L) compared with those of high phosphorus group (P<0.05), but no difference was found between 1 mmol/L magnesium intervention group and high phosphorus group. There were no significant differences in the ALP activity and Cbfα1 mRNA expression between intervention groups (2 and 3 mmol/L) and control group (P<0.05). The ALP activity and the expression of Cbfα1 mRNA were gradually decreased with the increased magnesium ion concentration in the inter-vention group, and which were lower than those of high phosphorus group (P<0.05). Conclusion Magnesium can reduce calcification and osteoblastic transdifferentiation, which may be achieved by reducing the expression of Cbfα1 in VSMCs.