临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
5期
467-470
,共4页
人肾母细胞瘤SK-NEP-1细胞%苦参碱%趋化因子受体4
人腎母細胞瘤SK-NEP-1細胞%苦參堿%趨化因子受體4
인신모세포류SK-NEP-1세포%고삼감%추화인자수체4
SK-NEP-1 cell%matrine%CXCR4
目的:探讨苦参碱对体外培养人肾母细胞瘤SK-NEP-1细胞抑制增殖及诱导凋亡的作用。方法体外培养人肾母细胞瘤SK-NEP-1细胞分对照组,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml苦参碱干预组,应用四甲基偶氮蓝比色法、流式细胞术检测苦参碱对SK-NEP-1细胞抑制率、凋亡率的影响,逆转录聚合酶链反应检测SK-NEP-1细胞的趋化因子受体4(CXCR4)mRNA表达。结果不同浓度苦参碱干预组及对照组SK-NEP-1细胞的抑制率、凋亡率以及CXCR4 mRNA表达差异均有统计学意义(P<0.01);随苦参碱浓度增加,SK-NEP-1细胞的抑制率和凋亡率呈上升趋势,而CXCR4 mRNA表达呈下降趋势,差异均有统计学意义(P<0.01)。结论苦参碱可以抑制SK-NEP-1细胞增殖并诱导凋亡,该作用可能与下调CXCR4的表达有关。
目的:探討苦參堿對體外培養人腎母細胞瘤SK-NEP-1細胞抑製增殖及誘導凋亡的作用。方法體外培養人腎母細胞瘤SK-NEP-1細胞分對照組,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml苦參堿榦預組,應用四甲基偶氮藍比色法、流式細胞術檢測苦參堿對SK-NEP-1細胞抑製率、凋亡率的影響,逆轉錄聚閤酶鏈反應檢測SK-NEP-1細胞的趨化因子受體4(CXCR4)mRNA錶達。結果不同濃度苦參堿榦預組及對照組SK-NEP-1細胞的抑製率、凋亡率以及CXCR4 mRNA錶達差異均有統計學意義(P<0.01);隨苦參堿濃度增加,SK-NEP-1細胞的抑製率和凋亡率呈上升趨勢,而CXCR4 mRNA錶達呈下降趨勢,差異均有統計學意義(P<0.01)。結論苦參堿可以抑製SK-NEP-1細胞增殖併誘導凋亡,該作用可能與下調CXCR4的錶達有關。
목적:탐토고삼감대체외배양인신모세포류SK-NEP-1세포억제증식급유도조망적작용。방법체외배양인신모세포류SK-NEP-1세포분대조조,0.5 mg/ml、1.0 mg/ml、1.5 mg/ml고삼감간예조,응용사갑기우담람비색법、류식세포술검측고삼감대SK-NEP-1세포억제솔、조망솔적영향,역전록취합매련반응검측SK-NEP-1세포적추화인자수체4(CXCR4)mRNA표체。결과불동농도고삼감간예조급대조조SK-NEP-1세포적억제솔、조망솔이급CXCR4 mRNA표체차이균유통계학의의(P<0.01);수고삼감농도증가,SK-NEP-1세포적억제솔화조망솔정상승추세,이CXCR4 mRNA표체정하강추세,차이균유통계학의의(P<0.01)。결론고삼감가이억제SK-NEP-1세포증식병유도조망,해작용가능여하조CXCR4적표체유관。
Objectives To investigate the effects of matrine on the proliferation and apoptosis of SK-NEP-1 cells in vitro, and its possible mechanism. Methods Trials were divided into following groups:control group, 0.5, 1.0 and 1.5mg/ml of ma-trine intervention groups. The inhibition rate of SK-NEP-1 cells treated with different concentration of Matrine was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry (FCM). RT-PCR analysis was employed to measure the PDCD4 mRNA expression. Results Matrine (final concentrations=0.5, 1.0 and 1.5mg/ml) could induce apoptosis and inhibit the growth of SK-NEP-1 cells. Compared with the controls without matrine treatment (8.81±3.71)%, the inhibition rates of SK-NEP-1 cells were (20.79 ± 6.20)%, (31.25 ± 5.07)%, and (51.15 ± 12.70)%, respectively;the apoptotic rates of SK-NEP-1 cells treated with different concentration of matrine were (13.67±0.78)%,(17.43±1.65)%and (20.80±1.54)%, respectively. Significant difference in the inhibition and apoptotic rates of SK-NEP-1 cells between each drug group and control group was observed(P<0.05), and the inhibition and apoptotic rates of SK-NEP-1 cells increased gradually with increased matrine concentration, thus exhibiting a dose-dependent effect(P<0.05). To the expression of CXCR4 mRNA,the grey levels of SK-NEP-1 cells treated with matrine intervention group (final concentrations=0.5, 1.0 and 1.5 mg/ml) were (0.720 ± 0.058), (0.540 ± 0.095) and (0.307 ± 0.050), respectively. The mRNA expression of CXCR4 was seen in SK-NEP-1 cells. Compared with control group, the expres-sion of CXCR4 mRNA was decreased significantly in matrine intervention group (P<0.01).There were significant difference in CXCR4 mRNA level among the SK-NEP-1 cells treated with 0.5,1.0,1.5mg/mL of matrine (P<0.01). Conclusions Matrine could induce apoptosis and inhibit the growth of SK-NEP-1 cells in a dose-dependent way which may be associated with the down-regulated CXCR4 expression in SK-NEP-1 cells.