检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
5期
540-544
,共5页
微小RNA-195%人膀胱癌细胞%细胞增殖%细胞迁移
微小RNA-195%人膀胱癌細胞%細胞增殖%細胞遷移
미소RNA-195%인방광암세포%세포증식%세포천이
Micro RNA-195%Human bladder cancer cell%Cell proliferation%Cell migration
目的：研究微小RNA（miRNA）-195对人膀胱癌细胞5637增殖、迁移及侵袭能力的影响。方法利用阳离子脂质体方法将miRNA-195转染至人膀胱癌细胞5637（miRNA-195组），同时以只转染病毒载体的5637细胞作为对照组。采用实时荧光定量聚合酶链反应（PCR）检测转染细胞中miRNA-195的表达量。采用噻唑蓝（MTT）法、细胞计数法和集落形成实验测定miRNA-195对5637细胞体外增殖的影响。采用划痕试验、Transwell实验、Matrigel 肿瘤细胞侵袭实验检测miRNA-195对5637细胞迁移、侵袭能力的影响。结果实时荧光定量PCR检测结果表明转染后的5637细胞高表达miRNA-195。MTT法、细胞计数法及集落形成实验证明miRNA-195可以抑制5637细胞在体外的增殖，miRNA-195组和对照组比较差异有统计学意义（P＜0．05）。划痕实验、Transwell实验以及Matrigel肿瘤细胞侵袭实验证明miRNA-195可以抑制5637细胞的迁移和侵袭能力，miRNA-195组和对照组比较差异有统计学意义（P＜0．05）。结论 miRNA-195能够抑制膀胱癌细胞5637在体外的增殖、迁移以及侵袭能力。
目的：研究微小RNA（miRNA）-195對人膀胱癌細胞5637增殖、遷移及侵襲能力的影響。方法利用暘離子脂質體方法將miRNA-195轉染至人膀胱癌細胞5637（miRNA-195組），同時以隻轉染病毒載體的5637細胞作為對照組。採用實時熒光定量聚閤酶鏈反應（PCR）檢測轉染細胞中miRNA-195的錶達量。採用噻唑藍（MTT）法、細胞計數法和集落形成實驗測定miRNA-195對5637細胞體外增殖的影響。採用劃痕試驗、Transwell實驗、Matrigel 腫瘤細胞侵襲實驗檢測miRNA-195對5637細胞遷移、侵襲能力的影響。結果實時熒光定量PCR檢測結果錶明轉染後的5637細胞高錶達miRNA-195。MTT法、細胞計數法及集落形成實驗證明miRNA-195可以抑製5637細胞在體外的增殖，miRNA-195組和對照組比較差異有統計學意義（P＜0．05）。劃痕實驗、Transwell實驗以及Matrigel腫瘤細胞侵襲實驗證明miRNA-195可以抑製5637細胞的遷移和侵襲能力，miRNA-195組和對照組比較差異有統計學意義（P＜0．05）。結論 miRNA-195能夠抑製膀胱癌細胞5637在體外的增殖、遷移以及侵襲能力。
목적：연구미소RNA（miRNA）-195대인방광암세포5637증식、천이급침습능력적영향。방법이용양리자지질체방법장miRNA-195전염지인방광암세포5637（miRNA-195조），동시이지전염병독재체적5637세포작위대조조。채용실시형광정량취합매련반응（PCR）검측전염세포중miRNA-195적표체량。채용새서람（MTT）법、세포계수법화집락형성실험측정miRNA-195대5637세포체외증식적영향。채용화흔시험、Transwell실험、Matrigel 종류세포침습실험검측miRNA-195대5637세포천이、침습능력적영향。결과실시형광정량PCR검측결과표명전염후적5637세포고표체miRNA-195。MTT법、세포계수법급집락형성실험증명miRNA-195가이억제5637세포재체외적증식，miRNA-195조화대조조비교차이유통계학의의（P＜0．05）。화흔실험、Transwell실험이급Matrigel종류세포침습실험증명miRNA-195가이억제5637세포적천이화침습능력，miRNA-195조화대조조비교차이유통계학의의（P＜0．05）。결론 miRNA-195능구억제방광암세포5637재체외적증식、천이이급침습능력。
Objective To investigate the influence of microRNA (miRNA)-195 on the proliferation,migration and invasion of human bladder cancer cell 5637.Methods Human bladder cancer cell 5637 was transfected with miRNA-195 by cationic liposomes as miRNA-195 group and transfected with the vector as control group.The expression of miRNA-195 was determined by real-time fluorescence quantitation polymerase chain reaction (PCR).Methyl thiazolyl tetrazolium(MTT)assay,cell counting assay and colony formation assay were used to detect the cell proliferation .Cell migration and invasion were determined by wound healling assay,Transwell assay and Matrigel tumour invasion assay. Results Real-time fluorescence quantitation PCR showed that there was high expression of miRNA-195 after transfection of cell 5637.MTT assay,cell counting assay and colony formation assay demonstrated that miRNA-195 can suppress cell 5637 proliferation in vitro,and there were statistical significance between miRNA-195 and control groups (P<0.05).Wound healing assay,Transwell assay and tumour invasion assay showed that miRNA-195 can suppress cell migration and invasion with statistical significance between the 2 groups(P<0.05 ).Conclusions MiRNA-195 has inhibitory ability on the proliferation,migration and invasion of bladder cancer cell 5637 cell.
