CAJ | 학술논문

目的：研究子痫前期患者血浆纤维蛋白原γ链浓度及其裂解产物，探讨其在子痫前期的预测和辅助诊断中的价值。方法全程追踪人绒毛膜促性腺激素（HCG）阳性孕妇至分娩后，收集临床资料和血浆标本，将31例子痫前期孕妇（轻度15例、重度16例）作为病例组，以31名正常妊娠孕妇作为对照组，应用免疫印迹法检测各组孕妇血浆纤维蛋白原γ链浓度。结果在正常妊娠过程中，纤维蛋白原γ链浓度差异无统计学意义（P＞0．05）。子痫前期患者妊娠32周（确诊前2～7周），血浆纤维蛋白原γ链浓度开始升高（P＜0．05），确诊时进一步升高。在16例子痫前期重度患者中，1例患者确诊前2周、另有3例患者确诊时出现了25000、27000及23000特异片段；15例轻度患者血浆中仅出现了1例；31名正常孕妇中，未见相关片段。结论子痫前期患者确诊前、后血浆纤维蛋白原合成及降解发生紊乱，γ链浓度显著增高，部分患者降解时出现25000、27000及23000特异片段。γ链浓度及这些特异片段在子痫前期的预测和辅助诊断中可能具有一定的价值。
목적：연구자간전기환자혈장섬유단백원γ련농도급기렬해산물，탐토기재자간전기적예측화보조진단중적개치。방법전정추종인융모막촉성선격소（HCG）양성잉부지분면후，수집림상자료화혈장표본，장31례자간전기잉부（경도15례、중도16례）작위병례조，이31명정상임신잉부작위대조조，응용면역인적법검측각조잉부혈장섬유단백원γ련농도。결과재정상임신과정중，섬유단백원γ련농도차이무통계학의의（P＞0．05）。자간전기환자임신32주（학진전2～7주），혈장섬유단백원γ련농도개시승고（P＜0．05），학진시진일보승고。재16례자간전기중도환자중，1례환자학진전2주、령유3례환자학진시출현료25000、27000급23000특이편단；15례경도환자혈장중부출현료1례；31명정상잉부중，미견상관편단。결론자간전기환자학진전、후혈장섬유단백원합성급강해발생문란，γ련농도현저증고，부분환자강해시출현25000、27000급23000특이편단。γ련농도급저사특이편단재자간전기적예측화보조진단중가능구유일정적개치。
Objective To investigate the levels of fibrinogenγand its products in plasma of preeclampsia pregnant women,and to study the predictive and aided diagnosis significance in preeclampsia.Methods The clinical data of human chorionic gonadotropin (HCG)-positive pregnant women were followed and collected until delivery,and the plasma specimens were collected.The levels of fibrinogen γwere measured by Western blotting in 3 1 patients with preeclampsia (15 patients with mild preeclampsia and 16 patients with severe preeclampsia)and 31 healthy controls being enrolled randomly.Results The levels of fibrinogenγhad no statistical significance in plasma of healthy controls during pregnancy (P >0.05 ).The levels of fibrinogen γin plasma of patients on 2-7 weeks before the onset of preeclampsia began to rise,and it rose more when preeclampsia happened(P<0.05).Three fibrinogen γfragments, 25 000,27 000 and 23 000,appeared in plasma of 1 patient on 2 weeks before preeclampsia happened and 3 patients when preeclampsia happened in 16 patients who developed severe preeclampsia.These fragments appeared in only 1 patient in 15 patients who developed mild preeclampsia.These fragments did not appear in 31 healthy controls. Conclusions Patients before and after the onset of preeclampsia have a synthesis and degradation fibrinogenγdisorder. The synthesis of fibrinogen γincreases,and fibrinogen γfragments,25 000,27 000 and 23 000,appear when fibrinogen γdegradated in some patients.The levels of fibrinogenγand these fragments may have some significance for predicting and diagnosing preeclampsia.