CAJ | 학술논문

基因敲除技术广泛应用于研究各类细胞和生物的基因功能。转录激活子样效应因子核酸酶技术（TALENs）是近年发展起来的一种新型基因敲除方法，其利用TALEs蛋白核酸结合域氨基酸序列与其靶位点核酸序列之间存在着恒定对应关系，从而组装出能特异性结合任意DNA序列的模块化蛋白。TALENs技术以其快速、高效的优势对生物基因研究领域产生深远影响，为遗传疾病的治疗提供了新的策略。
기인고제기술엄범응용우연구각류세포화생물적기인공능。전록격활자양효응인자핵산매기술（TALENs）시근년발전기래적일충신형기인고제방법，기이용TALEs단백핵산결합역안기산서렬여기파위점핵산서렬지간존재착항정대응관계，종이조장출능특이성결합임의DNA서렬적모괴화단백。TALENs기술이기쾌속、고효적우세대생물기인연구영역산생심원영향，위유전질병적치료제공료신적책략。
Gene knockout is a broadly applicable approach for efficiently editing any gene of cells and organisms. The transcription activator-like effector nuclease (TALEN)technique is a newly-developed method,and it can target essentially any sequence by comprising a nonspecific DNA-cleaving nuclease fused to a DNA-binding domain,which can be engineered easily.TALEN technique with the capability to quickly and efficiently alter genes have profound impacts on biological research and get potential therapeutic strategies for genetic diseases.