河北医药
河北醫藥
하북의약
HEBEI MEDICAL JOURNAL
2014年
7期
969-971
,共3页
郭立霞%徐育冬%王浩%朱明华
郭立霞%徐育鼕%王浩%硃明華
곽립하%서육동%왕호%주명화
佐剂性关节炎大鼠%可溶性重组人肿瘤坏死因子受体-抗体融合蛋白(益赛普)%肿瘤坏死因子-α%转化生长因子-β1%血管内皮生长因子
佐劑性關節炎大鼠%可溶性重組人腫瘤壞死因子受體-抗體融閤蛋白(益賽普)%腫瘤壞死因子-α%轉化生長因子-β1%血管內皮生長因子
좌제성관절염대서%가용성중조인종류배사인자수체-항체융합단백(익새보)%종류배사인자-α%전화생장인자-β1%혈관내피생장인자
adjuvant-induce-arthritis rat%rhu-TNFR-Fc%tumor necrosis factor-α%transforming growth factor-β1%vascular endothelial growth factor
目的：探讨肿瘤坏死因子受体-抗体融合蛋白对佐剂性关节炎大鼠滑膜中肿瘤坏死因子-α（ TNF-α）、转化生长因子-β1（TGF-β1）血管内皮生长因子（VEGF）表达的影响。方法将成年雄性Wistar大鼠随机分为5组，Ⅱ～Ⅴ组建立佐剂性关节炎大鼠（ AA）模型，于造模后第12天开始Ⅲ组给予甲氨喋呤灌胃治疗，Ⅳ～Ⅴ组给予益赛普皮下注射治疗。第28天取膝关节滑膜，常规HE染色，计算滑膜病理积分，免疫组织化学染色检测TNF-α、TGF-β1和VEGF在膝关节滑膜的表达。结果正常对照组大鼠滑膜组织均有少量TNF-α、TGF-β1和VEGF的表达，Ⅱ～Ⅴ组较Ⅰ组均明显增高（ P ＜0．05）。Ⅳ、Ⅴ组滑膜组织TNF-α表达较Ⅱ组明显减低（ P ＜0．05），而Ⅲ组与Ⅱ组无统计学意义（ P ＞0．05）。Ⅲ～Ⅴ组滑膜组织TGF-β1、VEGF的表达均明显低于Ⅱ组，差异有统计学意义（ P ＜0．05）。结论可溶性重组人肿瘤坏死因子受体-抗体融合蛋白可明显降低AA大鼠滑膜组织TNF-α、TGF-β1和VEGF表达，对AA大鼠的关节炎症有明显的治疗作用，可能抑制局部组织血管新生。
目的：探討腫瘤壞死因子受體-抗體融閤蛋白對佐劑性關節炎大鼠滑膜中腫瘤壞死因子-α（ TNF-α）、轉化生長因子-β1（TGF-β1）血管內皮生長因子（VEGF）錶達的影響。方法將成年雄性Wistar大鼠隨機分為5組，Ⅱ～Ⅴ組建立佐劑性關節炎大鼠（ AA）模型，于造模後第12天開始Ⅲ組給予甲氨喋呤灌胃治療，Ⅳ～Ⅴ組給予益賽普皮下註射治療。第28天取膝關節滑膜，常規HE染色，計算滑膜病理積分，免疫組織化學染色檢測TNF-α、TGF-β1和VEGF在膝關節滑膜的錶達。結果正常對照組大鼠滑膜組織均有少量TNF-α、TGF-β1和VEGF的錶達，Ⅱ～Ⅴ組較Ⅰ組均明顯增高（ P ＜0．05）。Ⅳ、Ⅴ組滑膜組織TNF-α錶達較Ⅱ組明顯減低（ P ＜0．05），而Ⅲ組與Ⅱ組無統計學意義（ P ＞0．05）。Ⅲ～Ⅴ組滑膜組織TGF-β1、VEGF的錶達均明顯低于Ⅱ組，差異有統計學意義（ P ＜0．05）。結論可溶性重組人腫瘤壞死因子受體-抗體融閤蛋白可明顯降低AA大鼠滑膜組織TNF-α、TGF-β1和VEGF錶達，對AA大鼠的關節炎癥有明顯的治療作用，可能抑製跼部組織血管新生。
목적：탐토종류배사인자수체-항체융합단백대좌제성관절염대서활막중종류배사인자-α（ TNF-α）、전화생장인자-β1（TGF-β1）혈관내피생장인자（VEGF）표체적영향。방법장성년웅성Wistar대서수궤분위5조，Ⅱ～Ⅴ조건립좌제성관절염대서（ AA）모형，우조모후제12천개시Ⅲ조급여갑안첩령관위치료，Ⅳ～Ⅴ조급여익새보피하주사치료。제28천취슬관절활막，상규HE염색，계산활막병리적분，면역조직화학염색검측TNF-α、TGF-β1화VEGF재슬관절활막적표체。결과정상대조조대서활막조직균유소량TNF-α、TGF-β1화VEGF적표체，Ⅱ～Ⅴ조교Ⅰ조균명현증고（ P ＜0．05）。Ⅳ、Ⅴ조활막조직TNF-α표체교Ⅱ조명현감저（ P ＜0．05），이Ⅲ조여Ⅱ조무통계학의의（ P ＞0．05）。Ⅲ～Ⅴ조활막조직TGF-β1、VEGF적표체균명현저우Ⅱ조，차이유통계학의의（ P ＜0．05）。결론가용성중조인종류배사인자수체-항체융합단백가명현강저AA대서활막조직TNF-α、TGF-β1화VEGF표체，대AA대서적관절염증유명현적치료작용，가능억제국부조직혈관신생。
Objective To investigate the effect of rhu-TNFR-Fc ( etanercept ) on the expression of TNF-α, TGF-β1 and VEGF in synovium of rats with adjuvant arthritis ( AA) .Methods The adult male Wistar rats were randomly divided into 5 groups:the rats in groupⅡ~Ⅴwere injected with Freund complete adjuvant to induce the experimental animal models with AA,on the 12th day after induction ,the rats in group Ⅲwere given MTX (2.7mg/kg.w) by gavage;the rats in groupⅣand group Ⅴwere given etanercept by subcutaneous injection .After 28 days,the synovium of joint of rats was taken out to be HE stained,then synovium pathological score was calculated ,and the expression levels of TNF-α,TGF-β1 and VEGF protein in synovium of knee joint were detected by histoimmunochemistry .Results The expression levels of TNF-α,TGF-β1 and VEGF in group Ⅱ~Ⅴwere significantly higher than those in groupⅠ( P <0.05),The expression levels of TNF-αin groupⅣand group Ⅴ were obviously decreased,as compared with those in group Ⅱ ( P <0.05),however,there were no significant differences between group Ⅲand group Ⅱ( P >0.05).The expression levels of TGF-β1 and VEGF in rats'synovium tissues in group Ⅲ~Ⅴwere significantly lower than those in group Ⅱ( P <0.05).Conclusion Etanercept can obviously reduce the expression levels of TNF-α, TGF-β1 and VEGF in rats'synovium tissues , which has obvious therapeutic effect on joint inflammation of rats with AA .which may inhibit blood vessel neogenesis in local tissues .
