CAJ | 학술논문

目的：研究淫羊藿苷（icariin，ICA）对钛（titanium，TI）颗粒作用下成骨细胞（osteoblast，OB）增殖和表型的影响。方法：体外培养新生大鼠颅骨成骨细胞，碱性磷酸酶（alkaline phosphatase，ALP）染色进行细胞鉴定。CCK-8法检测不同浓度钛颗粒对成骨细胞增殖能力的影响，并筛选出半数致死浓度。在此浓度作用下分别加入10-5、10-6、10-7、10-8、10-9、10-10 mol/L的淫羊藿苷进行干预，观察淫羊藿苷对钛颗粒作用下成骨细胞增殖能力的影响，找出最佳药物浓度。以最佳药物浓度干预钛颗粒作用下的成骨细胞，ELISA法检测各组细胞ALP活性，茜素红法钙化结节染色，观察药物对成骨细胞表型的影响。结果：不同浓度的钛颗粒均可抑制成骨细胞增殖（P＜0．05），0．5 mg/mL为钛颗粒的半数致死浓度。淫羊藿苷可以促进钛颗粒作用下的成骨细胞增殖（P＜0．05），最佳药物浓度为10-9 mol/L。在此浓度下的淫羊藿苷可以显著增加成骨细胞ALP活性（P＜0．05）和钙化结节形成。结论：淫羊藿苷可以促进钛颗粒作用下的成骨细胞增殖和表型表达，有望成为防治人工关节无菌性松动的药物。
목적：연구음양곽감（icariin，ICA）대태（titanium，TI）과립작용하성골세포（osteoblast，OB）증식화표형적영향。방법：체외배양신생대서로골성골세포，감성린산매（alkaline phosphatase，ALP）염색진행세포감정。CCK-8법검측불동농도태과립대성골세포증식능력적영향，병사선출반수치사농도。재차농도작용하분별가입10-5、10-6、10-7、10-8、10-9、10-10 mol/L적음양곽감진행간예，관찰음양곽감대태과립작용하성골세포증식능력적영향，조출최가약물농도。이최가약물농도간예태과립작용하적성골세포，ELISA법검측각조세포ALP활성，천소홍법개화결절염색，관찰약물대성골세포표형적영향。결과：불동농도적태과립균가억제성골세포증식（P＜0．05），0．5 mg/mL위태과립적반수치사농도。음양곽감가이촉진태과립작용하적성골세포증식（P＜0．05），최가약물농도위10-9 mol/L。재차농도하적음양곽감가이현저증가성골세포ALP활성（P＜0．05）화개화결절형성。결론：음양곽감가이촉진태과립작용하적성골세포증식화표형표체，유망성위방치인공관절무균성송동적약물。
Objective To study the effect of icariin on proliferation and phenotype of osteoblasts interfered with titanium particles. Methods Calvarial osteoblasts of newborn rats were cultured in vitro and cell identification was performed by alkaline phosphatase staining. Effect of titanium particles with different concentrations on osteoblasts proliferation was detected by CCK-8 method. Meanwhile , medium lethal concentration of titanium particles was screened out. Under this concentration , icariin with concentrations of 10-5, 10-6, 10-7, 10-8, 10-9 and 10-10 mol/L was added respectively for interference. The effect of icariin on proliferation of osteoblasts interfered with titanium particles was observed so as to obtain optimum drug concentration. Icariin with optimum concentraion was added into osteoblasts interfered with titanium particles. ALP activity of each group was detected by ELISA and calcified nodules were stained by alizarin S red to observe the effect of icariin on phenotype of osteoblasts. Results Titanium particles with different concentrations can inhibit proliferation of osteoblasts (P < 0.05), 0.5 mg/mL is its medium lethal concentration. Icariin can promote proliferation of osteoblasts interfered with particles (P < 0.05), the optimum drug concentration is 10-9 mol/L. With this concentration, icariin can significantly improve ALP activities of osteoblasts (P<0.05) and calcified nodules formation. Conclusion Icariin can promote proliferation and phenotypic expression of osteoblasts interfered with titanium particles. It may probably become an effective medicine for preventing aseptic joint prosthesis loosening.