CAJ | 학술논문

目的：观察siRNA沉默FRMD4A基因的表达对舌癌CAL-27细胞生物学行为的影响。方法：通过脂质体将FRMD4A-siRNA转染进CAL-27细胞，应用qRT-PCR检测转染后CAL-27细胞FRMD4A mRNA的表达情况，CCK-8检测其细胞增殖情况，流式细胞仪检测FRMD4A基因沉默后对CAL-27细胞周期分布的影响。结果：与对照组相比，FRMD4A-siRNA干扰组FRMD4A mRNA表达显著下降（94％），干扰组细胞增殖指数下降（P＜0．05），细胞周期出现G1期阻滞（P＜0．05）。结论：FRMD4A-siRNA能有效抑制舌癌CAL-27细胞FRMD4A mRNA的表达，并影响细胞周期分布，降低细胞增殖能力。
목적：관찰siRNA침묵FRMD4A기인적표체대설암CAL-27세포생물학행위적영향。방법：통과지질체장FRMD4A-siRNA전염진CAL-27세포，응용qRT-PCR검측전염후CAL-27세포FRMD4A mRNA적표체정황，CCK-8검측기세포증식정황，류식세포의검측FRMD4A기인침묵후대CAL-27세포주기분포적영향。결과：여대조조상비，FRMD4A-siRNA간우조FRMD4A mRNA표체현저하강（94％），간우조세포증식지수하강（P＜0．05），세포주기출현G1기조체（P＜0．05）。결론：FRMD4A-siRNA능유효억제설암CAL-27세포FRMD4A mRNA적표체，병영향세포주기분포，강저세포증식능력。
Objective To observe the influence of inactive FRMD4A gene′s expression on the biological behavior of tongue cancer CAL-27cell. Methods FRMD4A-siRNA was transfered into CAL-27 cell by lipidosome, to the expression of FRMD4A-siRNA in CAL-27 cell after transfection was detect by qRT-PCR cell proliferation , was checked by CCK-8,the influence of inactive FRMD4A gene on cell cycle distribution of CAL-27 cell was assayed by flow cytometry. Results Compared with the control group, FRMD4A mRNA expression significantly reduced in FRMD4A-siRNA interfering group (94%) and the cell proliferation index decreased(P<0.05). The cell cycle arrested in G1 period (P<0.05). Conclusion FRMD4A-siRNA could effectively inhibit FRMD4A mRNA expression in tongue cancer CAL-27cell, impact the distribution of cell cycle, and reduce cell proliferation.