CAJ | 학술논문

目的：探讨芦荟减阻剂对大鼠40％体表面积深Ⅱ度烧伤休克模型微循环的影响。方法：SD大鼠随机分为3组：对照组、减阻剂组及盐水组（烫伤后5 min给予减阻剂或者盐水进行补液复苏）。检测烧伤休克后24 h肺湿干重比（W/D）、病理及动脉血气；活体微循环观测技术观察烧伤休克后脊斜肌微动脉红细胞流速；观察动物的存活时间。结果：与对照组比较，盐水组肺组织W/D增加（P＜0．05），PO2降低、Lac增高（P＜0．05或P＜0．01），脊斜肌微动脉红细胞流速明显降低（P＜0．01），HE染色见大量中性粒细胞浸润；与盐水组比较，减阻剂组肺组织W/D明显降低（P＜0．05），PO2增加、Lac降低（P＜0．05或P＜0．01），HE染色中性粒细胞浸润减少，脊斜肌微动脉红细胞流速明显增高（P＜0．01）并且减阻剂治疗组大鼠生存时间显著延长（P＜0．05）。结论：芦荟减阻剂明显改善烧伤休克后肺损伤，其机制可能是通过改善烧伤休克后微循环。
목적：탐토호회감조제대대서40％체표면적심Ⅱ도소상휴극모형미순배적영향。방법：SD대서수궤분위3조：대조조、감조제조급염수조（탕상후5 min급여감조제혹자염수진행보액복소）。검측소상휴극후24 h폐습간중비（W/D）、병리급동맥혈기；활체미순배관측기술관찰소상휴극후척사기미동맥홍세포류속；관찰동물적존활시간。결과：여대조조비교，염수조폐조직W/D증가（P＜0．05），PO2강저、Lac증고（P＜0．05혹P＜0．01），척사기미동맥홍세포류속명현강저（P＜0．01），HE염색견대량중성립세포침윤；여염수조비교，감조제조폐조직W/D명현강저（P＜0．05），PO2증가、Lac강저（P＜0．05혹P＜0．01），HE염색중성립세포침윤감소，척사기미동맥홍세포류속명현증고（P＜0．01）병차감조제치료조대서생존시간현저연장（P＜0．05）。결론：호회감조제명현개선소상휴극후폐손상，기궤제가능시통과개선소상휴극후미순배。
Objective To investigate the effects of drag-reducing polymers on microcirculation in 40%total body surface area burn-injured rats. Methods SD rats were randomized into control group, drag-reducing polymer (DRPs) group and normal saline (NS) group (5 minutes after scald, drag-reducing polymer or saline was injected for fluid resuscitation). Wet dry weigh ratio of lung, histopathologic changes and arterial blood gas at 24 hour were respectively measured by wet dry weigh ratio method, hematoxylin-eosin (HE) staining and arterial blood gas analysis. The velocity of flow of red cell in oblique ridge and the survival time of burn-injured rats were observed. Results Compared with control group, rats in NS group exhibit significant lung injury characterized by a high W/D (P < 0.01), accumulation of a large number of neutrophils in HE stain, low partial pressure of oxygen (PO2) and high lactate (Lac) (P<0.05 or P<0.01) in arterial blood. Compared with the NS group, DRPs treatment rats exhibit significantly reduced lung injury characterized by W/D reducing (P < 0.05), the reduction of neutrophil infiltration, increased PO2, decreased Lac (P<0.05, P<0.01). In addition, DRPs treatment obviously increases the burn-induced low velocity of flow of red cell in oblique ridge (P<0.01). Moreover, the survival time of burned rats can be improved by DRPs treatment (P < 0.05). Conclusion DRPs ameliorates burn-induced acute lung injury, the mechanism may be through improving the burn-induced microcirculation disorders.