CAJ | 학술논문

目的：通过观察大鼠创伤性脑水肿后脑组织中核转录子-κB（ NF-κB）及白介素-1β（ IL-1β）的变化，探讨乌司他丁（ UTI）在大鼠创伤性脑水肿过程中对脑组织的保护作用及其机制。方法将30只健康成年SD大鼠随机分为正常对照组、创伤性脑水肿组及UTI干预组，每组10只。并于伤后8、24、72采用免疫组化法及血清ELISA法检测脑组织NF-κB和IL-1β的水平。结果创伤性脑水肿组和UTI组脑水肿部位NF-κB的活性和IL-1β的水平均较对照组明显升高（ P ＜0倐．01），且于24 h达到最高值，于72 h时有所下降；UTI组各时间点与创伤性脑水肿组比较差异均有统计学意义（ P ＜0．01）。结论乌司他丁对大鼠创伤性脑水肿引起的脑损伤具有明显的脑保护作用，通过抑制NF-κB和IL-1β信号传导通路是乌司他丁脑保护作用的重要机制之一。
목적：통과관찰대서창상성뇌수종후뇌조직중핵전록자-κB（ NF-κB）급백개소-1β（ IL-1β）적변화，탐토오사타정（ UTI）재대서창상성뇌수종과정중대뇌조직적보호작용급기궤제。방법장30지건강성년SD대서수궤분위정상대조조、창상성뇌수종조급UTI간예조，매조10지。병우상후8、24、72채용면역조화법급혈청ELISA법검측뇌조직NF-κB화IL-1β적수평。결과창상성뇌수종조화UTI조뇌수종부위NF-κB적활성화IL-1β적수평균교대조조명현승고（ P ＜0숙．01），차우24 h체도최고치，우72 h시유소하강；UTI조각시간점여창상성뇌수종조비교차이균유통계학의의（ P ＜0．01）。결론오사타정대대서창상성뇌수종인기적뇌손상구유명현적뇌보호작용，통과억제NF-κB화IL-1β신호전도통로시오사타정뇌보호작용적중요궤제지일。
Objective To observe the changes of expression levels of nuclear factor-κB ( NF-κB) and interleukin-1β( IL-1β) in rats with traumatic encephaledema in order to explore the protective effect and possible action mechanism of ulinastatin(UTI).Methods Thirty healthy adults SD 30 rats were randomly divided into three groups:normal control group, traumatic encephaledema group and UTI intervention group .The levels of NF-κB and IL-1βin serum and brain tissue homogenate (BTH) were detected by immunohistochemisty and enzyme-linked immunosorbent assay (ELISA) at the 8th, 12th,24th,72th hour after trauma.Results The levels of NF-κB and IL-1βin encephaledema position were significantly increased,as compared with those in control group ( P <0.01),which reached peak at the 24th hour,however,which reduced slightly at the 24th hour;there were significant differences in the changes of levels of NF-κB and IL-1βin every time point between UTI group and traumatic encephaledema group ( P <0.01).Conclusion UTI has obvious protective effect on the brain injury caused by traumatic encephaledema in rats , it may be one of the action mechanisms to inhibit the signal transduction way of NF-κB and IL-1β.