CAJ | 학술논문

目的：探讨变性梯度凝胶电泳（Denaturing gradient gel electrophoresis，DGGE）结合测序在检测混合细菌感染中的作用，为细菌多重感染的诊断提供新的思路。方法以3份临床培养鉴定为混合感染的胆囊穿刺引流液为对象，提取细菌总DNA并扩增16S rDNA-V3区，后进行DGGE分析，将所得条带切胶纯化再扩增，扩增产物进行测序及BLAST比对，比较比对结果与临床培养结果的一致性。结果 DGGE可以很好地分离16S rDNA-V3区混合PCR产物，每一个扩增子的测序比对结果与临床培养结果一致。结论 PCR-DGGE结合测序的方法可以很好地鉴定临床混合感染标本中的病原菌，可作为快速检测方法在临床应用。
목적：탐토변성제도응효전영（Denaturing gradient gel electrophoresis，DGGE）결합측서재검측혼합세균감염중적작용，위세균다중감염적진단제공신적사로。방법이3빈림상배양감정위혼합감염적담낭천자인류액위대상，제취세균총DNA병확증16S rDNA-V3구，후진행DGGE분석，장소득조대절효순화재확증，확증산물진행측서급BLAST비대，비교비대결과여림상배양결과적일치성。결과 DGGE가이흔호지분리16S rDNA-V3구혼합PCR산물，매일개확증자적측서비대결과여림상배양결과일치。결론 PCR-DGGE결합측서적방법가이흔호지감정림상혼합감염표본중적병원균，가작위쾌속검측방법재림상응용。
Objective To explore the value of PCR-DGGE and sequencing for detecting mixed bacte-rial infection. Methods A total of 3 samples which has been identified mixed bacterial infection by culture were included. The bacterial DNA was extracted and amplified the 16S rDNA-V3,following by DGGE , se-quencing and BLAST. Then, to compare the consistency of BLAST and cultivation. Results DGGE could differentiate the mixed amplicons of 16SrDNA-V3 sensationally, and the results of BLAST and cultivation were consistent. Conclusion PCR-DGGE and sequencing provide a new way to diagnose mixed bacterial infec-tion, which might be effective for identifying the pathogen in the sample.