CAJ | 학술논문

目的：建立高效液相色谱法测定芪白平肺胶囊中阿魏酸含量的方法，同时比较两种方法（水提和半仿生提取）提取芪白平肺胶囊中阿魏酸含量的有效性。方法采用 Welch Materials-C18（250 mm ×4．6 mm，5 mm）为色谱柱；以甲醇0．1％醋酸（33.67）混合溶液为流动相；流速1．0 mL/min；柱温35℃，检测波长321 nm。结果阿魏酸组与其它组分离良好，浓度在（0．0134～0．1340）mg/mL范围内，与峰面积呈良好的线性关系，相关系数为0．9998（n＝6），平均加样回收率（n＝6）为98．93％，相对标准差（RSD）为0．53％。结论该法简单，快速，灵敏度高，能够用于阿魏酸的含量测定，用SBE法提取芪白平肺胶囊阿魏酸含量优于 WE法提取。
목적：건립고효액상색보법측정기백평폐효낭중아위산함량적방법，동시비교량충방법（수제화반방생제취）제취기백평폐효낭중아위산함량적유효성。방법채용 Welch Materials-C18（250 mm ×4．6 mm，5 mm）위색보주；이갑순0．1％작산（33.67）혼합용액위류동상；류속1．0 mL/min；주온35℃，검측파장321 nm。결과아위산조여기타조분리량호，농도재（0．0134～0．1340）mg/mL범위내，여봉면적정량호적선성관계，상관계수위0．9998（n＝6），평균가양회수솔（n＝6）위98．93％，상대표준차（RSD）위0．53％。결론해법간단，쾌속，령민도고，능구용우아위산적함량측정，용SBE법제취기백평폐효낭아위산함량우우 WE법제취。
Objective To establish a quantitative method of ferulic acid by semi-bionic extraction ( SBE ) coupled with high performance liquid chromatography ,and compare the validity of ferulic acid in Qibaipingfei capsule by water extraction ( WE) method and SBE method .Methods The separation was carried out on a Welch-C18 column (250mm ×4.6mm,5μm).A mixture of methanol-1%(V/V)acetic acid solution(33.67)was used as the mobile phase at a flow rate of 1.0 mL/min in isocratic elution mode .The column temperature was kept at 35 °C, and the detection wavelength was set at 315 nm.Results The calibration curve was linear ( r =0.999 8, n =6 ) in the range of 0.013 4 ~0.134 0mg/mL of ferulic acid.The recoveries were 98.93% with RSDs of 0.53%. Conclusion On the basis of the advantages of simplicity and good reproducibility ,this method can be used for the determination of ferulic acid ,and also we can concluded that abstraction of Qibaipingfei capsule by SBE is better than WE method.