CAJ | 학술논문

目的：研究核转录因子-κB（NF-κB）p65和三叶因子3（TFF3）在胃癌组织中的表达及相关性，评价两者在胃癌发生、发展中的临床意义，以及与幽门螺杆菌（ Hp）感染的关系。方法采用免疫组化方法检测30例正常胃黏膜、32例不典型增生与70例胃癌组织中NF-κBp65和TFF3的表达情况，同时检测Hp感染情况。结果 NF-κBp65在正常胃黏膜、不典型增生、胃癌中表达水平呈递增强趋势（与正常胃黏膜组比较，χ2＝18．632、44．291，均P＜0．01），胃癌中表达最高，高于不典型增生组（χ2＝5．205，P＜0．05）。 TFF3表达水平亦呈递增强趋势（与正常胃黏膜组比较，χ2＝16．944、22．917，均P＜0．01），胃癌中表达最高，但与不典型增生之间差异无统计学意义（χ2＝0．162，P＞0．05）。不典型增生组织中Hp感染者NF-κBp65、TFF3表达显著高于Hp阴性病例（P＜0．01或P＜0．05）。胃癌组织中NF-κBp65表达与TFF3表达呈显著正相关（r＝0．350， P＝0．003）。结论 NF-κBp65、TFF3高表达可能是胃癌发生的早期事件，NF-κBp65和TFF3的异常表达呈正相关，两者在胃癌的发生、发展中可能存在协同作用。
목적：연구핵전록인자-κB（NF-κB）p65화삼협인자3（TFF3）재위암조직중적표체급상관성，평개량자재위암발생、발전중적림상의의，이급여유문라간균（ Hp）감염적관계。방법채용면역조화방법검측30례정상위점막、32례불전형증생여70례위암조직중NF-κBp65화TFF3적표체정황，동시검측Hp감염정황。결과 NF-κBp65재정상위점막、불전형증생、위암중표체수평정체증강추세（여정상위점막조비교，χ2＝18．632、44．291，균P＜0．01），위암중표체최고，고우불전형증생조（χ2＝5．205，P＜0．05）。 TFF3표체수평역정체증강추세（여정상위점막조비교，χ2＝16．944、22．917，균P＜0．01），위암중표체최고，단여불전형증생지간차이무통계학의의（χ2＝0．162，P＞0．05）。불전형증생조직중Hp감염자NF-κBp65、TFF3표체현저고우Hp음성병례（P＜0．01혹P＜0．05）。위암조직중NF-κBp65표체여TFF3표체정현저정상관（r＝0．350， P＝0．003）。결론 NF-κBp65、TFF3고표체가능시위암발생적조기사건，NF-κBp65화TFF3적이상표체정정상관，량자재위암적발생、발전중가능존재협동작용。
Objective To study the expressions and correlation of nuclear factor kappa B ( NF-B) p65 and trefoil factor family-3(TFF3) in gastric cancer,in order to evaluate the roles of NF-Bp65 and TFF3 in the occurrence and development of gastric cancer,and their correlation with Helicobacter pylori (Hp) infection.Methods The expressions of NF-Bp65 and TFF3 were detected by immunohistochemistey in 30 normal gastric mucosa specimens ,32 dysplasia specimens and 70 gastric cancer specimens .At the same time,the infection of Hp was detected .Results In normal gastric mucosa ,dysplasia and gastric cancer ,the expression of NF-Bp65 had a increasing tendency ( compared with the normal gastric mucosa group ,χ2 =18.632,44.291,all P<0.01).The expression of NF-Bp65 in gastric cancer group was higher than that in dysplasia group (χ2 =5.205,P<0.05).The expression of TFF3 in three groups also had a increasing tendency (compared with the normal gastric mucosa group ,χ2 =16.944,22.917,all P <0.01).The expression of TFF3 in gastric cancer group was higher than dysplasia group ,but there was no significant difference between the two groups (χ2 =0.162,P>0.05).In dysplasia group,the expressions of TFF3 and NF-κBp65 in Hp positive patients were significantly higher than those in Hp negative patients (P<0.01 or P<0.05).There was significantly positive correlation between expression of NF-Bp65 and TFF3 in gastric cancer group ( r=0.350,P=0.003).Conclusion High expressions of NF-Bp65 and TFF3 may be involved in an early event of gastric cancer . NF-Bp65 expression is positively correlated with the abnormal expression of TFF 3,both of which may be involved in the development and progression of gastric cancer .