CAJ | 학술논문

目的：检测系统性红斑狼疮（SLE）患者血清中载脂蛋白 E（ApoE）水平变化，探讨其临床意义。方法选取2012年8月至2013年5月于河北北方学院附属第二医院院进行诊治的47例S L E患者及40例健康者作为研究对象，应用酶联免疫吸附试验（ELISA）检测血清中ApoE水平，根据不同临床指标进行分组并进行统计学分析。结果与健康对照组相比，SLE患者组ApoE总体表达水平升高[（15．2±4．4）pg/mL vs ．（8．6±3．3） pg/mL ，P＜0．05]，其中活动组患者 ApoE较非活动组患者相比升高更为明显[（18．3±6．1）pg/mL vs ．（14．2 ± 5．7） pg/mL ，P＜0．05]；狼疮性肾炎组和非狼疮性肾炎组血清中 ApoE水平较健康对照组升高（P＜0．05），但两组间差异无统计学意义（P＞0．05）；抗双链DNA（ds-DNA）抗体阳性组患者血清中ApoE水平较抗ds-DNA抗体阴性组患者明显升高[（17．9 ± 5．8）pg/mL vs ．（13．9 ± 4．7） pg/mL ，P＜0．05]。结论 ApoE可作为反映SLE患者病情活动的有效指标，为疾病免疫治疗提供一定的理论依据。
목적：검측계통성홍반랑창（SLE）환자혈청중재지단백 E（ApoE）수평변화，탐토기림상의의。방법선취2012년8월지2013년5월우하북북방학원부속제이의원원진행진치적47례S L E환자급40례건강자작위연구대상，응용매련면역흡부시험（ELISA）검측혈청중ApoE수평，근거불동림상지표진행분조병진행통계학분석。결과여건강대조조상비，SLE환자조ApoE총체표체수평승고[（15．2±4．4）pg/mL vs ．（8．6±3．3） pg/mL ，P＜0．05]，기중활동조환자 ApoE교비활동조환자상비승고경위명현[（18．3±6．1）pg/mL vs ．（14．2 ± 5．7） pg/mL ，P＜0．05]；랑창성신염조화비랑창성신염조혈청중 ApoE수평교건강대조조승고（P＜0．05），단량조간차이무통계학의의（P＞0．05）；항쌍련DNA（ds-DNA）항체양성조환자혈청중ApoE수평교항ds-DNA항체음성조환자명현승고[（17．9 ± 5．8）pg/mL vs ．（13．9 ± 4．7） pg/mL ，P＜0．05]。결론 ApoE가작위반영SLE환자병정활동적유효지표，위질병면역치료제공일정적이론의거。
Objective To explore the expression level and clinical significance of apolipoprotein E (Apo E) in patients with systemic lupus erythematosus (SLE) .Methods From Aug .2012 to May 2013 ,47 SLE patients and 40 healthy controls in the Second Affiliated Hospital of Hebei North University were enrolled and detected for serum ApoE level by using enzyme linked immunosorbent assay (ELISA ) .Patients were divided into different groups ac-cording to clinical indicators ,and data were statistically analyzed .Results Compared with healthy controls ,ApoE level of SLE patients were increased [(15 .2 ± 4 .4)pg/mL vs .(8 .6 ± 3 .3)pg/mL ,P<0 .05)] ,and activity SLE pa-tients higher more pronounced than inactivity [(18 .3 ± 6 .1)pg/mL vs .(14 .2 ± 5 .7)pg/mL ,P<0 .05] .ApoE levels of patients with or without nephritis were higher than healthy controls (P<0 .05) ,but no statistically different be-tween the two patient groups (P> 0 .05) .ApoE of anti-dsDNA antibody positive SLE patients were marked in-creased ,compared with anti-dsDNA antibody negative patients [(17 .9 ± 5 .8)pg/mL vs .(13 .9 ± 4 .7)pg/mL ,P<0 .05)] .Conclusion ApoE could be used as an effective indicator of disease activity in patients with SLE ,and provide certain theoretical basis for immunotherapy of disease .