检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
10期
1341-1343,1346
,共4页
HIV1+2型抗体%金标层析法%酶联免疫吸附试验%免疫印迹
HIV1+2型抗體%金標層析法%酶聯免疫吸附試驗%免疫印跡
HIV1+2형항체%금표층석법%매련면역흡부시험%면역인적
HIV1+2 antibody%GICA%ELISA%Western blot
目的：对金标层析法（GICA）试剂和酶联免疫吸附试验（ELISA）试剂初筛 HIV1＋2进行对比分析，评估GICA试剂的品质，并将筛查出阳性血清进行免疫印迹试验（WB）确认，探讨筛查结果与WB试验结果的关系。方法选用市面上口碑较好的5个厂家的艾滋病病毒（HIV ）抗体GICA快速筛查试剂与ELISA HIV抗体初筛试剂平行检测16780例血清标本，初筛阳性血清送疾控中心用WB试验做确认，对WB试验结果不确定者进行4、8周随访检测，8周随访标本补充HIV-1病毒载量检测。结果7种试剂敏感性为99．23％～100．00％，其中3种GI-CA试剂在敏感性上已经达到ELISA试剂的水平（100％），假阳性率为0．08％～0．12％，功效率均在99．80％以上，282例初筛阳性血清经WB法确认有260例为阳性，5例不确定血清经随访检测确定均为阴性，初筛试剂阳性率可达92．20％。结论 GICA检测试剂筛检HIV抗体检测敏感性、特异性和功效率均较高，某些厂家生产的试剂在敏感性、特异性方面已经达到了ELISA试剂的水平，且快速、简便、价廉，是理想的HIV抗体筛查试剂。
目的：對金標層析法（GICA）試劑和酶聯免疫吸附試驗（ELISA）試劑初篩 HIV1＋2進行對比分析，評估GICA試劑的品質，併將篩查齣暘性血清進行免疫印跡試驗（WB）確認，探討篩查結果與WB試驗結果的關繫。方法選用市麵上口碑較好的5箇廠傢的艾滋病病毒（HIV ）抗體GICA快速篩查試劑與ELISA HIV抗體初篩試劑平行檢測16780例血清標本，初篩暘性血清送疾控中心用WB試驗做確認，對WB試驗結果不確定者進行4、8週隨訪檢測，8週隨訪標本補充HIV-1病毒載量檢測。結果7種試劑敏感性為99．23％～100．00％，其中3種GI-CA試劑在敏感性上已經達到ELISA試劑的水平（100％），假暘性率為0．08％～0．12％，功效率均在99．80％以上，282例初篩暘性血清經WB法確認有260例為暘性，5例不確定血清經隨訪檢測確定均為陰性，初篩試劑暘性率可達92．20％。結論 GICA檢測試劑篩檢HIV抗體檢測敏感性、特異性和功效率均較高，某些廠傢生產的試劑在敏感性、特異性方麵已經達到瞭ELISA試劑的水平，且快速、簡便、價廉，是理想的HIV抗體篩查試劑。
목적：대금표층석법（GICA）시제화매련면역흡부시험（ELISA）시제초사 HIV1＋2진행대비분석，평고GICA시제적품질，병장사사출양성혈청진행면역인적시험（WB）학인，탐토사사결과여WB시험결과적관계。방법선용시면상구비교호적5개엄가적애자병병독（HIV ）항체GICA쾌속사사시제여ELISA HIV항체초사시제평행검측16780례혈청표본，초사양성혈청송질공중심용WB시험주학인，대WB시험결과불학정자진행4、8주수방검측，8주수방표본보충HIV-1병독재량검측。결과7충시제민감성위99．23％～100．00％，기중3충GI-CA시제재민감성상이경체도ELISA시제적수평（100％），가양성솔위0．08％～0．12％，공효솔균재99．80％이상，282례초사양성혈청경WB법학인유260례위양성，5례불학정혈청경수방검측학정균위음성，초사시제양성솔가체92．20％。결론 GICA검측시제사검HIV항체검측민감성、특이성화공효솔균교고，모사엄가생산적시제재민감성、특이성방면이경체도료ELISA시제적수평，차쾌속、간편、개렴，시이상적HIV항체사사시제。
Objective To compare the performance of colloidal gold immunochromatographic (GICA)and en-zyme linked immunosorbent assay (ELISA) for the detection of human immunodeficiency virus 1+2 (HIV1+2) an-tibody .Methods A total of 16 780 serum samples were tested detected by GICA kits and ELISA kit of 5 suppliers . The positive samples were then confirmed by western blot (WB) assay .Those with indefinite results of WB detection were re-tested 4 and 8 weeks later ,and HIV-1 viral load testing(VL)was detected at the 8th week .Results Sensitivi-ties of the 7 sets of kits were 99 .23% -100 .00% ,including 3 sets of GICA kit with sensitivity of 100% as ELISA kits ,false positive rates of 0 .08% -0 .12% and effective rates higher than 99 .80% .In 282 samples with positive re-sult of primary screening ,260 cases were confirmed to be positive .Five samples with indefinite results of WB detec-tion were confirmed to be negative by follow -up detection .The positive rate of primary screening was 92 .20% .Con-clusion The sensitivity ,specificity and effective rates of GICA reagents could be high ,and performance of kits from some suppliers could reach the level of ELISA kits ,and GICA detection might be fast ,easy to operate and with low cost ,which could be ideal reagents for screening HIV antibody .
