中国动物保健
中國動物保健
중국동물보건
CHINA ANIMAL HEALTH
2014年
5期
16-18
,共3页
张漫%李娜%高木珍%张宁%刘晓辉%王辉
張漫%李娜%高木珍%張寧%劉曉輝%王輝
장만%리나%고목진%장저%류효휘%왕휘
氟喹诺酮类药物%达氟沙星%恩诺沙星%环丙沙星%沙拉沙星%残留检测
氟喹諾酮類藥物%達氟沙星%恩諾沙星%環丙沙星%沙拉沙星%殘留檢測
불규낙동류약물%체불사성%은낙사성%배병사성%사랍사성%잔류검측
fluoroquinolones%danofloxacin%enrofloxacin%ciprofloxacin%sarafloxacin%residues determination
随机选取本实验室内的8名化验员，分别测定猪肉组织中氟喹诺酮类药物残留量，通过对结果数据的分析，得出本实验室测定结果的准确程度。通过磷酸盐缓冲溶液提取猪肉组织中的氟喹诺酮类药物残留，经C18柱净化后，用高效液相色谱法测定含量。环丙沙星、达氟沙星、恩诺沙星和沙拉沙星的平均回收率（%）分别为：95.6、86.9、89.3和95.6，批内变异系数均≤14%，批间变异系数均≤6%。结果数据满足标准要求，证明本实验室能准确开展此项检测活动。
隨機選取本實驗室內的8名化驗員，分彆測定豬肉組織中氟喹諾酮類藥物殘留量，通過對結果數據的分析，得齣本實驗室測定結果的準確程度。通過燐痠鹽緩遲溶液提取豬肉組織中的氟喹諾酮類藥物殘留，經C18柱淨化後，用高效液相色譜法測定含量。環丙沙星、達氟沙星、恩諾沙星和沙拉沙星的平均迴收率（%）分彆為：95.6、86.9、89.3和95.6，批內變異繫數均≤14%，批間變異繫數均≤6%。結果數據滿足標準要求，證明本實驗室能準確開展此項檢測活動。
수궤선취본실험실내적8명화험원，분별측정저육조직중불규낙동류약물잔류량，통과대결과수거적분석，득출본실험실측정결과적준학정도。통과린산염완충용액제취저육조직중적불규낙동류약물잔류，경C18주정화후，용고효액상색보법측정함량。배병사성、체불사성、은낙사성화사랍사성적평균회수솔（%）분별위：95.6、86.9、89.3화95.6，비내변이계수균≤14%，비간변이계수균≤6%。결과수거만족표준요구，증명본실험실능준학개전차항검측활동。
We selecting 8 technicians in our laboratory randomly, letting they determine fluoroquinolones residues in pork tissues separately. Based on the analysis of the experimental data, we concluded the accuracy of my determination results. The Fluoroquinolones residues are extracted with phosphate buffer solution, cleaned by C18 SPE cartridge, then content was determined by HPLC. The average recoveries of four components were 95.6%, 86.9%, 89.3%, 95.6%.The variation coefficient of inter-assay were below 14%, intra-assay were below 6%. The results meet requirements , so we can carry out the test.
