CAJ | 학술논문

植物中单糖的提取从传统的固-液萃取发展到超声萃取、微波辅助萃取等，实现了样品和溶剂用量少、萃取速度快、绿色环保等目的。纯化过程通常使用固相萃取柱，其填料从商业化的C18柱、NH2柱和离子交换柱，到分子印迹固相萃取柱、离子液体优化的萃取柱，结构和功能上不断完善。因单糖多数不具有紫外吸收和荧光性质，在分离检测中，人们采取了多种方法来解决这一问题，以满足色谱分析的需要。本文综述了植物中单糖的样品前处理方法及色谱分析方法。针对单糖提取、纯化、分离检测存在的问题以及解决方案进行了论述，并对植物中单糖的色谱分析方法的发展进行了展望。
식물중단당적제취종전통적고-액췌취발전도초성췌취、미파보조췌취등，실현료양품화용제용량소、췌취속도쾌、록색배보등목적。순화과정통상사용고상췌취주，기전료종상업화적C18주、NH2주화리자교환주，도분자인적고상췌취주、리자액체우화적췌취주，결구화공능상불단완선。인단당다수불구유자외흡수화형광성질，재분리검측중，인문채취료다충방법래해결저일문제，이만족색보분석적수요。본문종술료식물중단당적양품전처리방법급색보분석방법。침대단당제취、순화、분리검측존재적문제이급해결방안진행료논술，병대식물중단당적색보분석방법적발전진행료전망。
The extraction methods of saccharides from plants were developed from solid-liquid extraction toultrasonic extraction and microwave assisted extraction, and achieved the results of sample and solvent con-sumption, high speed and environment friendly. The solid phase extraction was adopted into purification process, and its packings have being modified all the way on structures and functions, the materials variedfrom C18, NH2,ion exchange column to molecularly imprinting materials and optimized ionic liquid column. As to detec-tion, different methods were developed for monosaccharides to meet the requirements of chromatographic analysis, because most of them leak UV absorption and fluorescence properties. In this paper, the development of sample pretreatment methods and chromatographic analysis of monosaccharides in the plant samples was reviewed. The extraction, purification, separation and detection of monosaccharides from different samples were discussed.