CAJ | 학술논문

为探究骨炭修复剂对Pb和Cd复合污染土壤中生物活性的修复作用及其随时间变化的效果，在温室条件下通过盆栽实验，研究骨炭修复对土壤酶（脲酶、脱氢酶、酸性磷酸酶）活性与土壤微生物生物量碳含量的影响，测定种植的青菜体内抗氧化酶（过氧化物酶、过氧化氢酶）活性与膜脂过氧化产物丙二醛含量以及青菜生物量的变化。研究结果表明，骨炭修复后，土壤微生物生物量碳含量与对照组相比提高0.33~0.46倍，同一处理下随培养时间的增加，脲酶和脱氢酶活性的变化减小，骨炭的修复效果逐渐减弱；修复后地上部分青菜的生物量是对照组的2.05~4.56倍，青菜体内的过氧化氢酶活性和丙二醛均有明显升高的过程，骨炭对其的修复效果减弱。以上结果显示：骨炭修复能够有效地增强土壤中生物的活性，显著提高作物的产量；在添加时间较短时，骨炭对污染的土壤-植物系统的修复效果更好；而土壤中的脲酶和脱氢酶、植物中的丙二醛能较好指示骨炭的修复作用。
위탐구골탄수복제대Pb화Cd복합오염토양중생물활성적수복작용급기수시간변화적효과，재온실조건하통과분재실험，연구골탄수복대토양매（뇨매、탈경매、산성린산매）활성여토양미생물생물량탄함량적영향，측정충식적청채체내항양화매（과양화물매、과양화경매）활성여막지과양화산물병이철함량이급청채생물량적변화。연구결과표명，골탄수복후，토양미생물생물량탄함량여대조조상비제고0.33~0.46배，동일처리하수배양시간적증가，뇨매화탈경매활성적변화감소，골탄적수복효과축점감약；수복후지상부분청채적생물량시대조조적2.05~4.56배，청채체내적과양화경매활성화병이철균유명현승고적과정，골탄대기적수복효과감약。이상결과현시：골탄수복능구유효지증강토양중생물적활성，현저제고작물적산량；재첨가시간교단시，골탄대오염적토양-식물계통적수복효과경호；이토양중적뇨매화탈경매、식물중적병이철능교호지시골탄적수복작용。
Bone char has shown to reduce the availability of heavy metals in soils, thus improving biological activities. Here a pot experiment was designed to explore the effects of bone char applications on biological activities in soil and plant under Pb and Cd combined pollution. Soil microbial biomass carbon, soil enzymes(urease, dehydrogenase, and acid phosphatase), plant enzymes(peroxidase, catalase, malondi-aldehyde)of Brassica Campestris and plant dry biomass were measured at different times. The results showed that additions of bone char in-creased soil microbial biomass by 33%~46%as compared to the control. The enhancing effects of bone char on soil urease and dehydroge-nase became smaller as incubation time increased. Dry biomass of Brassica campestris in bone char treatments was as 2.05~4.56 times much as that in the control. Plant catalase activity and malondialdehyde contents increased significantly in later incubation period. These re-sults indicate that bone char could restore biological activities of polluted soil and increase the production of crops, with a better effective-ness in short term. Soil urease and dehydrogenase and plant malondialdehyde could be better indicators of restoration of combined-heavy metal polluted soil by bone char.