CAJ | 학술논문

从江苏省南通市某农药厂的活性污泥中分离出一株苯胺降解菌株耘圆，根据表型特征、生理生化特性和员远杂则阅晕粤序列分析，将其鉴定为红球菌属（Rhodococc us sp.）的细菌。菌株E2降解苯胺的最适温度为30℃、最适pH为7.0，降解苯胺的最高浓度为800mg·L-1。该菌能降解苯胺、邻甲基苯胺、对甲基苯胺和2,5，一二氯苯胺，与已报道的苯胺降解菌的底物谱显著不同。通过扩增高度保守的片段和染色体步移，获得了菌株E2中负责苯胺降解的基因簇，该基因簇在基因组成、排布及同源性方面和已报道的基因簇有较大差异，是研究苯胺降解分子机理的较好材料。
종강소성남통시모농약엄적활성오니중분리출일주분알강해균주운원，근거표형특정、생리생화특성화원원잡칙열훈월서렬분석，장기감정위홍구균속（Rhodococc us sp.）적세균。균주E2강해분알적최괄온도위30℃、최괄pH위7.0，강해분알적최고농도위800mg·L-1。해균능강해분알、린갑기분알、대갑기분알화2,5，일이록분알，여이보도적분알강해균적저물보현저불동。통과확증고도보수적편단화염색체보이，획득료균주E2중부책분알강해적기인족，해기인족재기인조성、배포급동원성방면화이보도적기인족유교대차이，시연구분알강해분자궤리적교호재료。
Aniline and its derivatives have become environmental pollutants. The existing aniline-degrading bacteria have narrow substrate spectrum toward aniline derivatives and can degrade parts of these compounds. Here we isolated an aniline-degrading bacterium E2 from activated sludge originated from an insecticide factory, and revealed its molecular nature. The strain E2 was identified as Rhodococcus sp. based on morphological, physiological and biochemical properties, and homology analysis of 16S rDNA sequence. The optimum temperature and pH value for the degradation by the strain E2 were 30℃and 7.0, respectively. The highest aniline concentration at which the strain E2 could degrade was 800 mg·L-1. It could degrade aniline, o-Toluidine, p-Toluidine and 2,5-dichloroaniline. Aniline-degrading gene cluster was cloned from the strain E2 by amplifying the highly conserved fragment and chromosome walking. This cluster was significantly different from the current ones in composition, arrangement and homology of genes. It would be a valuable material for investigating the molecular mechanisms of aniline degradation.