中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
6期
30-32,61
,共4页
毛立梅%戴建华%张晏苗%包蔚雷%高园园%朱春富%张京平
毛立梅%戴建華%張晏苗%包蔚雷%高園園%硃春富%張京平
모립매%대건화%장안묘%포위뢰%고완완%주춘부%장경평
胆固醇结石%胆囊%模型%小鼠
膽固醇結石%膽囊%模型%小鼠
담고순결석%담낭%모형%소서
Cholesterol gallstone%Gallbladder%Model%Mouse
目的：建立简单、可靠、高效的小鼠胆囊胆固醇结石模型，为研究胆石成因及防治提供重要手段。方法 C57BL/6小鼠随机分为对照组和模型组，对照组喂饲基础饲料，模型组喂饲致石饲料（基础饲料加10%猪油、1%胆固醇及0.5%胆酸）。两组小鼠分别于喂养4周和8周后，计算小鼠存活率，同时各取一半数量小鼠在乙醚吸入麻醉下手术取胆囊及血液标本，分别检测成石率、血脂浓度及胆汁胆固醇饱和度。结果对照组小鼠8周存活率100%，模型组小鼠死亡1只，存活率95%。对照组8周成石率为零，模型组4周成石率80%，8周成石率100%。血脂分析表明，与对照组比较，模型组4周和8周总胆固醇及低密度脂蛋白显著升高（P＜0.01），甘油三酯浓度轻度升高（P＜0.05），高密度脂蛋白显著降低（P＜0.01）。4周和8周时胆汁胆固醇饱和度测定，对照组分别为0.48±0.29和0.58±0.21，模型组分别为1.36±0.36和1.52±0.37，模型组胆固醇浓度处于过饱和状态。结论本模型方法简单、成石率高、动物死亡率低，可作为研究胆石成因及防治的备选模型。
目的：建立簡單、可靠、高效的小鼠膽囊膽固醇結石模型，為研究膽石成因及防治提供重要手段。方法 C57BL/6小鼠隨機分為對照組和模型組，對照組餵飼基礎飼料，模型組餵飼緻石飼料（基礎飼料加10%豬油、1%膽固醇及0.5%膽痠）。兩組小鼠分彆于餵養4週和8週後，計算小鼠存活率，同時各取一半數量小鼠在乙醚吸入痳醉下手術取膽囊及血液標本，分彆檢測成石率、血脂濃度及膽汁膽固醇飽和度。結果對照組小鼠8週存活率100%，模型組小鼠死亡1隻，存活率95%。對照組8週成石率為零，模型組4週成石率80%，8週成石率100%。血脂分析錶明，與對照組比較，模型組4週和8週總膽固醇及低密度脂蛋白顯著升高（P＜0.01），甘油三酯濃度輕度升高（P＜0.05），高密度脂蛋白顯著降低（P＜0.01）。4週和8週時膽汁膽固醇飽和度測定，對照組分彆為0.48±0.29和0.58±0.21，模型組分彆為1.36±0.36和1.52±0.37，模型組膽固醇濃度處于過飽和狀態。結論本模型方法簡單、成石率高、動物死亡率低，可作為研究膽石成因及防治的備選模型。
목적：건립간단、가고、고효적소서담낭담고순결석모형，위연구담석성인급방치제공중요수단。방법 C57BL/6소서수궤분위대조조화모형조，대조조위사기출사료，모형조위사치석사료（기출사료가10%저유、1%담고순급0.5%담산）。량조소서분별우위양4주화8주후，계산소서존활솔，동시각취일반수량소서재을미흡입마취하수술취담낭급혈액표본，분별검측성석솔、혈지농도급담즙담고순포화도。결과대조조소서8주존활솔100%，모형조소서사망1지，존활솔95%。대조조8주성석솔위령，모형조4주성석솔80%，8주성석솔100%。혈지분석표명，여대조조비교，모형조4주화8주총담고순급저밀도지단백현저승고（P＜0.01），감유삼지농도경도승고（P＜0.05），고밀도지단백현저강저（P＜0.01）。4주화8주시담즙담고순포화도측정，대조조분별위0.48±0.29화0.58±0.21，모형조분별위1.36±0.36화1.52±0.37，모형조담고순농도처우과포화상태。결론본모형방법간단、성석솔고、동물사망솔저，가작위연구담석성인급방치적비선모형。
Objective To establish a mouse model of cholesterol gallstone by lithogenic diet. Methods C57BL/6 mice were randomly divided into control group and experimental group. Mice of control group were fed with normal diet and those of experimental group were fed with lithogenic diet,which included 10% fat,1% cholesterol and 0.5%cholic acid. After 4-and 8-week's feeding, mice were sacrificed respectively and specimens were collected for further assay. Results The survival of mice was 100%in control group and 95%in experimental group in the end of 8-week's feeding.In control group,there was no gallbladder cholesterol stone formation.In experimental group,the rate of stone formation was 80% in week 4 and 100% in week 8, respectively. Blood lipid analysis indicated that the level of total cholesterol and low-density lipoprotein cholesterol were significantly higher in experimental group than those in control group in both week 4 and week 8 (P<0.01).On the other hand, the level of blood triglyceride in experimental group was slightly higher versus that in control group (P<0.05) and high-density lipoprotein cholesterol remarkably lower versus that in control group (P < 0.01). Bile analysis showed that super saturation of bile cholesterol in experiment group happened but no super saturation in control group. Conclusion It is a good method reported in this paper for the establishment of C57BL/6 mouse model of cholesterol gallstone by lithogenic diet.
