中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
3期
197-200
,共4页
李惠%冯素英%林麟%周武庆%徐浩翔%李志量%靳培英
李惠%馮素英%林麟%週武慶%徐浩翔%李誌量%靳培英
리혜%풍소영%림린%주무경%서호상%리지량%근배영
角蛋白细胞%桥粒芯糖蛋白质1%桥粒芯糖蛋白质3
角蛋白細胞%橋粒芯糖蛋白質1%橋粒芯糖蛋白質3
각단백세포%교립심당단백질1%교립심당단백질3
Keratinocytes%Desmoglein 1%Desmoglein 3
目的 测定不同类型角质形成细胞(KC)桥粒芯糖蛋白(DSG)1、DSG3及DSG1 mRNA、DSG3mRNA表达.方法 不同KC(HaCaT细胞、A431细胞及原代KC)培养后,直接免疫荧光观察细胞DSG1、DSG3的表达,定量聚合酶链反应(qPCR)测定DSG1 mRNA、DSG3 mRNA相对表达水平.结果 DSG1与DSG3表达于三种角质形成细胞,荧光强度显示,A431细胞高于HaCaT细胞,HaCaT细胞高于原代KC.三种细胞均表达DSG1、DSG3 mRNA,原代KC DSG1与DSG3 mRNA的相对表达量分别为HaCaT细胞的291.7％及237.4％,差异有统计学意义(P＜0.01)；A431细胞DSG1 mRNA为HaCaT细胞的0.1％、DSG3 mRNA则为HaCaT细胞的18.8％,差异有统计学意义(P＜0.05).结论 三种KC均可用于对DSG1、DSG3的相关研究；相对而言,正常培养的A431细胞表面DSG1、DSG3表达较HaCaT细胞及原代KC高；原代KC DSG1、DSG3mRNA水平较HaCaT细胞及A431细胞高.
目的 測定不同類型角質形成細胞(KC)橋粒芯糖蛋白(DSG)1、DSG3及DSG1 mRNA、DSG3mRNA錶達.方法 不同KC(HaCaT細胞、A431細胞及原代KC)培養後,直接免疫熒光觀察細胞DSG1、DSG3的錶達,定量聚閤酶鏈反應(qPCR)測定DSG1 mRNA、DSG3 mRNA相對錶達水平.結果 DSG1與DSG3錶達于三種角質形成細胞,熒光彊度顯示,A431細胞高于HaCaT細胞,HaCaT細胞高于原代KC.三種細胞均錶達DSG1、DSG3 mRNA,原代KC DSG1與DSG3 mRNA的相對錶達量分彆為HaCaT細胞的291.7％及237.4％,差異有統計學意義(P＜0.01)；A431細胞DSG1 mRNA為HaCaT細胞的0.1％、DSG3 mRNA則為HaCaT細胞的18.8％,差異有統計學意義(P＜0.05).結論 三種KC均可用于對DSG1、DSG3的相關研究；相對而言,正常培養的A431細胞錶麵DSG1、DSG3錶達較HaCaT細胞及原代KC高；原代KC DSG1、DSG3mRNA水平較HaCaT細胞及A431細胞高.
목적 측정불동류형각질형성세포(KC)교립심당단백(DSG)1、DSG3급DSG1 mRNA、DSG3mRNA표체.방법 불동KC(HaCaT세포、A431세포급원대KC)배양후,직접면역형광관찰세포DSG1、DSG3적표체,정량취합매련반응(qPCR)측정DSG1 mRNA、DSG3 mRNA상대표체수평.결과 DSG1여DSG3표체우삼충각질형성세포,형광강도현시,A431세포고우HaCaT세포,HaCaT세포고우원대KC.삼충세포균표체DSG1、DSG3 mRNA,원대KC DSG1여DSG3 mRNA적상대표체량분별위HaCaT세포적291.7％급237.4％,차이유통계학의의(P＜0.01)；A431세포DSG1 mRNA위HaCaT세포적0.1％、DSG3 mRNA칙위HaCaT세포적18.8％,차이유통계학의의(P＜0.05).결론 삼충KC균가용우대DSG1、DSG3적상관연구；상대이언,정상배양적A431세포표면DSG1、DSG3표체교HaCaT세포급원대KC고；원대KC DSG1、DSG3mRNA수평교HaCaT세포급A431세포고.
Objective To detect the mRNA and protein expressions of desmoglein 1 (DSG1) and DSG3 in different types of keratinocytes (KCs).Methods Two keratinocyte cell lines HaCaT and A431,as well as primary keratinocytes from human abdomenal skin served as the object of this study.Direct immunofluorescence assay was performed to observe and quantify the expressions of DSG1 and DSG3,and quantitative PCR (qPCR) to determine the mRNA expressions of DSG1 and DSG3,in these cells.Results Both DSG1 and DSG3 were expressed in all the three types of keratinocytes,and the fluorescence intensity of DSG1 and DSG3 in HaCaT cells was higher than that in primary keratinocytes but lower than that in A431 cells.Similarly,all the keratinocytes expressed DSG1 and DSG3 mRNA,with the relative expression levels of DSG1 and DSG3 mRNA in primary keratinocytes being 291.7％ and 237.4％ of those in HaCaT cells respectively (both P ＜ 0.01),and those in A431 cells being 0.1％ and 18.8％ of those in HaCaT cells respectively (both P ＜ 0.05).Conclusions HaCaT cells,A431 cells and primary keratinocytes all can be used for the study of DSG1 and DSG3,of which,A431 cells show the strongest expressions of DSG1 and DSG3,and primary keratinocytes display the highest expressions of DSG1 and DSG3 mRNAs.
