临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2014年
10期
777-781
,共5页
大鼠%缺血再灌注损伤%乌司他丁%自由基
大鼠%缺血再灌註損傷%烏司他丁%自由基
대서%결혈재관주손상%오사타정%자유기
Rats%Ischemia-reperfusion injury%Ulinastatin%Free radicals
目的:探讨乌司他丁对大鼠骨骼肌缺血再灌注损伤的保护作用及其机制。方法将72只SPF级大鼠随机分为3组:对照组、缺血再灌注组及缺血再灌注-乌司他丁组,每组24只。各组根据再灌注时间点不同分为0 h、2 h、4 h、8 h,各时间点6只大鼠。建立下肢缺血/再灌注模型:对照组、缺血再灌注组、缺血再灌注-乌司他丁组。同时,经股静脉采血备检;取骨骼肌组织分别固定,备电镜、光镜检验。分别测定各组动物骨骼肌在发生再灌注时间点0h、2h、4 h、8 h时血浆中乳酸脱氢酶( LDH)、肌酸激酶( CK)的含量。同时检测各组不同时点大鼠后肢腓肠肌组织超氧化物歧化酶( SOD)及丙二醛( MDA)的含量。光镜下观察骨骼肌细胞结构形态。结果缺血再灌注组血浆LDH、CK的含量4 h达高峰,之后开始下降,缺血再灌注组和乌司他丁组LDH、CK在各时间点的水平含量明显高于对照组( P <0.05),但乌司他丁组各不同时点血浆LDH、CK的含量明显低于缺血再灌注组( P <0.05);缺血再灌注组腓肠肌组织表现为SOD活性均低于对照组( P <0.05),其中以4 h为最低;脂质过氧化产物MDA 含量均高于对照组( P <0.05),且以4 h为最高;缺血再灌注组各组动物血浆SOD 含量均低于对照组( P <0.05);乌司他丁组各时间点腓肠肌中MDA含量低于缺血再灌注组( P <0.05),SOD含量高于缺血再灌注组。结论①乌司他丁可以减轻缺血再灌注损伤;②乌司他丁能有效抑制大鼠下肢缺血再灌注损伤时骨骼肌细胞破坏而引起的LDH和CK的释放,因此对大鼠肢体缺血再灌注骨骼肌的损伤有保护作用;③在缺血再灌注损伤时,乌司他丁组的SOD活性要高于单纯缺血再灌注组,而反映氧自由基对骨骼肌损害的MDA的含量却较单纯缺血再灌注低,提示乌司他丁在清除氧自由基及减轻后肢缺血再灌注损伤方面有明显保护作用。
目的:探討烏司他丁對大鼠骨骼肌缺血再灌註損傷的保護作用及其機製。方法將72隻SPF級大鼠隨機分為3組:對照組、缺血再灌註組及缺血再灌註-烏司他丁組,每組24隻。各組根據再灌註時間點不同分為0 h、2 h、4 h、8 h,各時間點6隻大鼠。建立下肢缺血/再灌註模型:對照組、缺血再灌註組、缺血再灌註-烏司他丁組。同時,經股靜脈採血備檢;取骨骼肌組織分彆固定,備電鏡、光鏡檢驗。分彆測定各組動物骨骼肌在髮生再灌註時間點0h、2h、4 h、8 h時血漿中乳痠脫氫酶( LDH)、肌痠激酶( CK)的含量。同時檢測各組不同時點大鼠後肢腓腸肌組織超氧化物歧化酶( SOD)及丙二醛( MDA)的含量。光鏡下觀察骨骼肌細胞結構形態。結果缺血再灌註組血漿LDH、CK的含量4 h達高峰,之後開始下降,缺血再灌註組和烏司他丁組LDH、CK在各時間點的水平含量明顯高于對照組( P <0.05),但烏司他丁組各不同時點血漿LDH、CK的含量明顯低于缺血再灌註組( P <0.05);缺血再灌註組腓腸肌組織錶現為SOD活性均低于對照組( P <0.05),其中以4 h為最低;脂質過氧化產物MDA 含量均高于對照組( P <0.05),且以4 h為最高;缺血再灌註組各組動物血漿SOD 含量均低于對照組( P <0.05);烏司他丁組各時間點腓腸肌中MDA含量低于缺血再灌註組( P <0.05),SOD含量高于缺血再灌註組。結論①烏司他丁可以減輕缺血再灌註損傷;②烏司他丁能有效抑製大鼠下肢缺血再灌註損傷時骨骼肌細胞破壞而引起的LDH和CK的釋放,因此對大鼠肢體缺血再灌註骨骼肌的損傷有保護作用;③在缺血再灌註損傷時,烏司他丁組的SOD活性要高于單純缺血再灌註組,而反映氧自由基對骨骼肌損害的MDA的含量卻較單純缺血再灌註低,提示烏司他丁在清除氧自由基及減輕後肢缺血再灌註損傷方麵有明顯保護作用。
목적:탐토오사타정대대서골격기결혈재관주손상적보호작용급기궤제。방법장72지SPF급대서수궤분위3조:대조조、결혈재관주조급결혈재관주-오사타정조,매조24지。각조근거재관주시간점불동분위0 h、2 h、4 h、8 h,각시간점6지대서。건립하지결혈/재관주모형:대조조、결혈재관주조、결혈재관주-오사타정조。동시,경고정맥채혈비검;취골격기조직분별고정,비전경、광경검험。분별측정각조동물골격기재발생재관주시간점0h、2h、4 h、8 h시혈장중유산탈경매( LDH)、기산격매( CK)적함량。동시검측각조불동시점대서후지비장기조직초양화물기화매( SOD)급병이철( MDA)적함량。광경하관찰골격기세포결구형태。결과결혈재관주조혈장LDH、CK적함량4 h체고봉,지후개시하강,결혈재관주조화오사타정조LDH、CK재각시간점적수평함량명현고우대조조( P <0.05),단오사타정조각불동시점혈장LDH、CK적함량명현저우결혈재관주조( P <0.05);결혈재관주조비장기조직표현위SOD활성균저우대조조( P <0.05),기중이4 h위최저;지질과양화산물MDA 함량균고우대조조( P <0.05),차이4 h위최고;결혈재관주조각조동물혈장SOD 함량균저우대조조( P <0.05);오사타정조각시간점비장기중MDA함량저우결혈재관주조( P <0.05),SOD함량고우결혈재관주조。결론①오사타정가이감경결혈재관주손상;②오사타정능유효억제대서하지결혈재관주손상시골격기세포파배이인기적LDH화CK적석방,인차대대서지체결혈재관주골격기적손상유보호작용;③재결혈재관주손상시,오사타정조적SOD활성요고우단순결혈재관주조,이반영양자유기대골격기손해적MDA적함량각교단순결혈재관주저,제시오사타정재청제양자유기급감경후지결혈재관주손상방면유명현보호작용。
Objective To investigate the protective effect and mechanism of ulinastatin in rat skeletal muscle ischemia-reperfusion inju-ry. Methods Lower limb ischemia-reperfusion model was established in SPF grade rats. Seventy-two SPF grade rats were randomly divided in-to three groups:control group(n=24),ischemia-reperfusion group(n=24)and ischemia-reperfusion-ulinastatin(IRU)group(n=24). According to reperfusion time,each group was further divided into 0 h,2 h,4 h,8 h subgroups with six rats in each subgroup. Plasma levels of LDH and CK,and SOD and MDA content in gastrocnemius muscle were detected in each group and subgroup. Morphology of skeletal muscle cells was observed in light microscope. Results Plasma levels of LDH and CK reached a peak at 4 h and then began to decline in ischemia-reperfu-sion group. LDH and CK levels at each time point were significantly higher( P <0. 05)in ischemia-reperfusion group and IRU group than con-trol group;however they were significantly lower in IRU group than ischemia-reperfusion group( P <0. 05). Compared with control group,in ischemia-reperfusion group SOD activity of the gastrocnemius muscle was lower( P <0. 05),and it was lowest in 4 h subgroup;MDA content was higher( P <0. 05)and it was highest in 4 h subgroup;and plasma SOD level was lower( P <0. 05). In IRU group gastrocnemius MDA content was lower( P <0. 05),but SOD content was higher than ischemia-reperfusion group at each time point. Conclusion ①Ulinastatin can reduce ischemia-reperfusion injury;②Ulinastatin can effectively inhibit the release of LDH and CK,so it may has a protective effect on ische-mia-reperfusion injury of skeletal muscle in rat;③The observation that SOD activity is higher,but MDA content is lower in IRU group than is-chemia-reperfusion group indicates the effects of ulinastatin on scavenging oxygen free radicals and reduce ischemia-reperfusion injury.