CAJ | 학술논문

目的：探讨小肠切除术（MSBR）后 Notch信号通路表达与肠隐窝上皮细胞增殖之间的关系。方法40只雄性 SD大鼠随机分组，接受 MSBR手术（70％小肠中段切除）或假手术。手术后7 d，收集肠道组织标本，行逆转录-PCR（RT-PCR）、蛋白免疫印迹法（Western blot）实验，观察肠黏膜 Notch信号通路蛋白（mRNA）及蛋白表达变化；并制作组织学切片，免疫组织化学方法进一步观察 Notch信号通路蛋白在肠黏膜的表达变化及表达部位。培养大鼠肠道隐窝上皮细胞 IEC-6，给予重组大鼠Jagged-1，噻唑蓝（MTT）实验观察重组人鼠Jagged-1对 IEC-6细胞增殖的影响。结果 MSBR手术促进了大鼠肠黏膜Jagged-1、Notch-1、Hes-1 mRNA及蛋白的表达；免疫组织化学结果显示，表达升高的Jagged-1、NICD、Hes-1蛋白共定位于大鼠肠道隐窝上皮细胞；体外培养 IEC-6细胞，MTT实验进一步明确，Jagged-1具有促进肠上皮细胞增殖的作用。结论 Notch信号通路在 MSBR术后大鼠肠黏膜中促进了肠隐窝上皮细胞的增殖，参与了 MSBR术后的肠适应过程。
목적：탐토소장절제술（MSBR）후 Notch신호통로표체여장은와상피세포증식지간적관계。방법40지웅성 SD대서수궤분조，접수 MSBR수술（70％소장중단절제）혹가수술。수술후7 d，수집장도조직표본，행역전록-PCR（RT-PCR）、단백면역인적법（Western blot）실험，관찰장점막 Notch신호통로단백（mRNA）급단백표체변화；병제작조직학절편，면역조직화학방법진일보관찰 Notch신호통로단백재장점막적표체변화급표체부위。배양대서장도은와상피세포 IEC-6，급여중조대서Jagged-1，새서람（MTT）실험관찰중조인서Jagged-1대 IEC-6세포증식적영향。결과 MSBR수술촉진료대서장점막Jagged-1、Notch-1、Hes-1 mRNA급단백적표체；면역조직화학결과현시，표체승고적Jagged-1、NICD、Hes-1단백공정위우대서장도은와상피세포；체외배양 IEC-6세포，MTT실험진일보명학，Jagged-1구유촉진장상피세포증식적작용。결론 Notch신호통로재 MSBR술후대서장점막중촉진료장은와상피세포적증식，삼여료 MSBR술후적장괄응과정。
Objective To investigate the role of Notch signaling in massive small bowel resection(MSBR)-associated crypt epi-thelial cell proliferation.Methods Male Sprague-Dawley rats were subj ected to sham operation(bowel transection and reanastomo-sis)or 70 % mid-SBR.Intestinal tissue samples were collected at 7 d after operation.The expression of Notch pathway mRNAs and proteins were analyzed by RT-PCR and Western blot.The expression of the Notch pathway proteins Jagged-1,NICD and Hes-1 also determined through immunohistochemical.Furthermore,IEC-6 cells were used to examine the function of the Jagged-1 signaling system.Results SBR increased crypt epithelial cell proliferation and increased expression of Jagged-1 and Hes-1 mRNA and protein along with cleaved Notch-1.Immunohistochemical staining showed that Jagged-1,cleaved Notch-1 and Hes-1 colocalized in the same crypt epithelial cell population.Recombinant Jagged-1 significantly stimulated the proliferation of IEC-6 cells.Conclusion The Jag-ged-1/Notch-1/Hes-1 signaling pathway is involve in intestinal adaptation through increase crypt epithelial cell proliferation.